Acinetobacter: A Tale of Two Genera

  • Elliot Juni
Part of the Basic Life Sciences book series


The name Acinetobacter was first applied in 1954 by Brisou and Prévot (5) to nonmotile strains of Achromobacter, the latter group of bacteria representing an earlier classification of gram-negative saprophytes that could be distinguished from other similar bacteria because of their lack of pigmentation (16). In subsequent studies of acinetobacters it was shown that some of them were oxidase-positive and others were oxidase-negative (7). As a result of several investigations of the oxidase-negative acinetobacters an official designation was made of these organisms as species of Acinetobacter calcoaceticus (26). The oxidase-positive acinetobacters were then shown to be unrelated to the oxidase-negative acinetobacters (22) and can no longer be considered strains of the genus Acinetobacter. Both groups of acinetobacters contain members that can be manipulated genetically and each will be considered in this discussion.


Acinetobacter Calcoaceticus Transformation Assay Transduce Bacteriophage Acinetobacter Strain Official Designation 
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  1. 1.
    Ahliquist, E.F., C.A. Fewson, D.A. Ritchie, J. Podmore, and V. Rowell. Competence for genetic transformation in Acinetobacter calcoaceticus NCIB 8250. FEMS Microbiol. Letter, 7: 107–109 (1980).Google Scholar
  2. 2.
    Al-Khoja, M.S., and J.H. Darrell. The skin as the source of Acinetobacter and Moraxella species occurring in blood cultures. J. Clin. Pathol. 32: 497–499 (1979).CrossRefGoogle Scholar
  3. 3.
    Baumann, P. Isolation of Acinetobacter from soil and water. J. Bacteriol. 96: 39–42 (1968).Google Scholar
  4. 4.
    Baumann, P., M. Doudoroff, and R.Y. Stanier. A study of the Moraxella group. II. Oxidase-negative species (Genus Acinetobacter). J. Bacteriol. 95: 1520–1541 (1968).Google Scholar
  5. 5.
    Brisou, J., and A.R. Prevot. Etudes de systematique bacterienne. X. Revision des espaces réunies dans le genre Achromobacter. Ann. Inst. Pasteur (Paris) 86: 722–728 (1954).Google Scholar
  6. 6.
    Brooks, K. and T. Sodeman. Clinical studies on a transformation test for identification of Acinetobacter (Mima and Herellea). Appl. Microbiol. 27: 1023–1026 (1974).Google Scholar
  7. 7.
    Buttiaux, R., and P. Gagnon. Au sujet de la classification des Pseudomonas et des Achromobacter. Ann. Inst. Pasteur (Lille) 10: 121–149 (1959).Google Scholar
  8. 8.
    Carter, G.R., T.T. Isoun, and K.K. Keahey. Occurrence of Mima and Herellea species in clinical specimens from various animals. J. Amer. Vet. Med. Assoc. 156: 1313–1318 (1970).Google Scholar
  9. 9.
    Cruze, J.A., J.T. Singer, and W.R. Finnerty. Conditions for quantitative transformation in Acinetobacter calcoaceticus. Current Microbiol. 3: 129–132 (1980).CrossRefGoogle Scholar
  10. 10.
    Ditta, G., S. Stanfield, D. Corbin, and D.R. Helinski. Broad host range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77: 7347–7351 (1980).CrossRefGoogle Scholar
  11. 11.
    Glew, R.H., R.C. Moellering, and L.J. Kunz. Infections with Acinetobacter calcoaceticus (Herellea vaginicola): Clinical and laboratory studies. Medicine 56: 79–97 (1977).CrossRefGoogle Scholar
  12. 12.
    Henriksen, S.D. Moraxella, Acinetobacter and the Mimeae. Bacteriol. Rev. 37: 522–561 (1973).Google Scholar
  13. 13.
    Herman, N.J. and E. Juni. Isolation and characterization of a generalized transducing bacteriophage for Acinetobacter. J. Virol. 13: 46–52 (1974).Google Scholar
  14. 14.
    Hinchliffe, E. and A. Vivian. Naturally occurring plasmids in Acinetobacter calcoaceticus: A P class R factor of restricted host range. J. Gen. Microbiol. 116: 75–80 (1980).Google Scholar
  15. 15.
    Hinchliffe, E. and A. Vivian. Gene transfer in Acinetobacter calcoaceticus: Fertility variants of the sex factor pAV1. J. Gen. Microbiol. 119: 117–122 (1980).Google Scholar
  16. 16.
    Ingram, M., and J.M. Shewan. Introductory reflections on the Pseudomonas-Achromobacter group. J. Appl. Bacteriol. 23: 373–378 (1960).CrossRefGoogle Scholar
  17. 17.
    Ito, H., T. Sato, and H. Iizuka. Study of the intermediate type of Moraxella and Acinetobacter occurring in radurized Vienna sausages. Agr. Biol. Chem. 40: 867–873 (1976).CrossRefGoogle Scholar
  18. 18.
    Juni, E. The bacterial oxidation of acetoin. Bacteriol. Proc. pg. 140 (1952).Google Scholar
  19. 19.
    Juni, E. Interspecies transformation of Acinetobacter: Genetic evidence for a ubiquitous genus. J. Bacteriol 112: 917–931 (1972).Google Scholar
  20. 20.
    Juni, E. Genetics and physiology of Acinetobacter. Ann. Rev. Microbiol. 32: 349–371 (1978).CrossRefGoogle Scholar
  21. 21.
    Juni, E. and G.A. Heym. A cyclic pathway for the bacterial dissimilation of 2,3-butanediol, acetylmethylcarbinol, and diacetyl. I. General aspects of the 2,3-butanediol cycle. J. Bacteriol. 71: 425–432 (1956).Google Scholar
  22. 22.
    Juni, E., and G. A. Heym. Transformation assay for identification of psychrotrophic achromobacters. Appl. Environ. Microbiol. 40: 1106–1114 (1980).Google Scholar
  23. 23.
    Juni, E., and A. Janik. Transformation of Acinetobacter calcoaceticus (Bacterium anitratum). J. Bacteriol. 98: 281–288 (1969).Google Scholar
  24. 24.
    Laycock, R.A. and L.W. Regier. Psudomonads and achromobacters in the spoilage of irradiated haddock of different preirradiation quality. Appl. Microbiol. 20: 333–341 (1970).Google Scholar
  25. 25.
    Lemoigne, M., H. Girard, and G. Jacobelli. Bacteria du sol utilisant facilement le 2,3 butanediol. Ann. Inst. Pasteur (Paris) 82: 389–398 (1952).Google Scholar
  26. 26.
    Lessel, E.F. International Committee on Nomenclature of Bacteria, Subcommittee on the Taxonomy of Moraxella and Allied Bacteria, Minutes of the Meeting, 11 August 1970. Inst. J. Syst. Bacteriol. 21: 213–214 (1971)CrossRefGoogle Scholar
  27. 27.
    Marcus, B.B., S.B. Samuels, B. Pittman, W.B. Cherry. A serologic study of Herellea vaginicola and its identification by immunofluorescent staining. Amer. J. Clin. Pathol. 52: 309–319 (1969).Google Scholar
  28. 28.
    Olsen, R.H. and Shipley, P. Host range and properties of the Pseudomonas aeruginosa R factor R1822. J. Bacteriol. 113: 772–780 (1973).Google Scholar
  29. 29.
    Sawula, R.V. and I.P. Crawford. Mapping of the tryptophan genes of Acinetobacter calcoaceticus by transformation. J. Bacteriol. 112: 797–805 (1972).Google Scholar
  30. 30.
    Stewart, J.E., R.E. Kallio, D.P. Stevenson, A.C. Jones, and D. O. Schissler. Bacterial hydrocarbon oxidation. I. Oxidation of n-hexadecane by a gram-negative coccus. J. Bacteriol. 78: 441–448 (1959).Google Scholar
  31. 31.
    Towner, K.J. Chromosome mapping in Acinetobacter calcoaceticus. J. Gen. Microbiol. 104: 175–180 (1978).Google Scholar
  32. 32.
    Towner, K.J. and A. Vivian. RP4-mediated conjugation in Acinetobacter calcoaceticus. J. Gen. Microbiol. 93: 355–360 (1976).Google Scholar
  33. 33.
    Towner, K.J. and A. Vivian. RP4 fertility variants in Acinetobacter calcoaceticus. Genet. Res. Camb. 28: 301–306 (1976).CrossRefGoogle Scholar
  34. 34.
    Towner, K.J. and A. Vivian. Plasmids capable of transfer and chromosome mobilization in Acinetobacter calcoaceticus. J. Gen. Microbiol. 101: 167–171 (1977).Google Scholar
  35. 35.
    Twarog, R. and L.E. Blouse. Isolation and characterization of transducing bacteriophage BP1 for Bacterium anitratum (Achromobacter sp.). J. Virol. 2: 716–722 (1968).Google Scholar
  36. 36.
    Warskow, A.L., and E. Juni. Nutritional requirements of Acinetobacter strains isolated from soil, water, and sewage. J. Bacteriol. 112: 1014–1016 (1972).Google Scholar
  37. 37.
    Weitzman, P.D.J., H.A. Kinghorn, L.J. Beecroft, and S. Harford. Mutant citrate synthases from Acinetobacter generated by transformation. Biochem. Soc. Trans. 6: 436–438 (1978).Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Elliot Juni
    • 1
  1. 1.The University of MichiganAnn ArborUSA

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