Strategies for Cloning in Bacillus Subtilis

  • James A. Hoch
  • Ahn Nguyen
  • Eugenio Ferrari
Part of the Basic Life Sciences book series


In the past few years we have been involved in attempts to generate a versatile cloning system for Bacillus subtilis. We have had many failures and a few successes. It is our hope that the successful strategies will be applicable to a wide variety of microorganisms.


Bacillus Subtilis Chloramphenicol Resistance Temperate Bacteriophage Partial Methylation EcoRI Digestion 
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  1. 1.
    Bolivar, R., R.L. Roderigues, P.J. Greene, M.C. Betlach, H.L. Heyneker and H.W. Boyer. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2: 95–113 (1977).CrossRefGoogle Scholar
  2. 2.
    Chang, S. and S.N. Cohen. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Molec. gen. Genet. 168: 111–115 (1979).CrossRefGoogle Scholar
  3. 3.
    Ehrlich, S.D. Replication and expression of plasmids from Staphyloccus aureus in Bacillus subtilis. Proc. Natl. Acad. Sci., USA 74: 1680–1682 (1977).CrossRefGoogle Scholar
  4. 4.
    Ehrlich, S.D. DNA cloning in Bacillus subtilis. Proc. Natl. Acad. Sci., USA 75: 1433–1436, (1978).CrossRefGoogle Scholar
  5. 5.
    Ferrari, E., D.J. Henner and J.A. Hoch. Isolation of Bacillus subtilis genes from a Charon 4A library. J. Bacteriol. 146: 430–432 (1981).Google Scholar
  6. 6.
    Gryczan, T., S. Contente and D. Dubnau. Molecular cloning of heterologous chromosomal DNA by recombination between plasmid vector and a homologous resident plasmid in Bacillus subtilis. Molec. gen. Genet. 177: 459–467 (1980).CrossRefGoogle Scholar
  7. 7.
    Gryczan, T. and D. Dubnau. Construction and properties of chimeric plasmids in Bacillus subtilis. Proc. Natl. Acad.Google Scholar
  8. 8.
    Haldenwang, W.G., C.D.B. Banner, J.F. Ollington, R. Losick, J.A. Hoch, M.B. O’Connor and A.L. Sonenshein. Mapping a cloned gene under sporulation control by insertion of a drug resistance marker into the Bacillus subtilis chromosome. J. Bacteriol. 142: 90–98 (1980).Google Scholar
  9. 9.
    Henner, D.J. and J.A. Hoch. The Bacillus subtilis chromosome. Microbiol. Rev. 44: 57–82 (1980).Google Scholar
  10. 10.
    Keggins, K.M., P.S. Lovett and E.J. Duvall. Molecular cloning of genetically active fragments of Bacillus DNA in Bacillus subtilis and properties of the vector plasmid pUB110. Proc. Natl. Acad. Sci., USA 75: 1423–1427 (1978).CrossRefGoogle Scholar
  11. 11.
    Soberon, X., L. Covarrubias and F. Bolivar. Construction and characterization of new cloning vehicles. IV. Deletion and derivatives of pBR322 and pBR325. Gene 9: 287–305 (1980).CrossRefGoogle Scholar
  12. 12.
    Wilson, R.E., J.A. Hoch and K. Bott. Genetic mapping of a linked cluster of ribosomal RNA genes in Bacillus subtilis. J. Bacteriol. in press.Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • James A. Hoch
    • 1
  • Ahn Nguyen
    • 1
  • Eugenio Ferrari
    • 1
  1. 1.Research Institute of Scripps ClinicLa JollaUSA

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