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The Regulation of Yeast Gene Expression by Multiple Control Elements

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Genetic Engineering of Microorganisms for Chemicals

Part of the book series: Basic Life Sciences ((BLSC))

Abstract

The effective employment of biological systems for production of commercially useful chemicals often requires a thorough understanding of how the desired system is regulated. In the past we have looked to procaryotic systems for models of how control is exerted; they have served us well. However, it is becoming abundantly clear that the procaryotic paradigm does not extend to eucaryotic systems. For these, new models must be developed and new paradigms constructed. My purpose is the development of such a model in the simple eucaryotic microorganism, Saccharomyces cerevisiae. This yeast is perhaps the oldest commercially cultured organism in existence. It is also one of the most well studied genetic systems available. Our model is the metabolism of two main nitrogen sources, allantoin and arginine. We selected two systems instead of one to gain some insight into the spectrum of regulatory mechanisms that operate in eucaryotic cells. Our choice also presents the added opportunity of assessing the means used to integrate the expression of separately controlled, but functionally related genes.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, 15260, USA

    Terrance G. Cooper

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  1. Terrance G. Cooper
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Editors and Affiliations

  1. Associated Universities, Inc., Washington, D.C., USA

    Alexander Hollaender

  2. Department of Microbiology, University of Illinois, Champaign, Urbana, USA

    Ralph D. DeMoss

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© 1982 Plenum Press, New York

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Cooper, T.G. (1982). The Regulation of Yeast Gene Expression by Multiple Control Elements. In: Hollaender, A., DeMoss, R.D., Kaplan, S., Konisky, J., Savage, D., Wolfe, R.S. (eds) Genetic Engineering of Microorganisms for Chemicals. Basic Life Sciences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4142-0_14

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  • DOI: https://doi.org/10.1007/978-1-4684-4142-0_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4144-4

  • Online ISBN: 978-1-4684-4142-0

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