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Transformation of Neurospora Crassa Utilizing Recombinant Plasmid DNA

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Genetic Engineering of Microorganisms for Chemicals

Part of the book series: Basic Life Sciences ((BLSC))

Abstract

In comparison to yeast (15), the development of recombinant DNA technology for filamentous fungi is in its early stages. Recombinant DNA technology is dependent on two different though related matters: an efficient transformation system for the organism and an appropriate vector. In addition, transformation in any organism involves two processes: a system which permits the uptake of DNA into the cell and the subsequent integration of the DNA into a chromosome or its maintenance as a self-replicating entity in the recipient strain. An efficient transformation system for Neurospora crassa has been developed utilizing a cloned gene from Neurospora (4,27). The development of this procedure will be described here with the hope that some of the methods will be applicable to the development of such systems for other filamentous fungi.

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Author information

Authors and Affiliations

  1. Department of Molecular and Population Genetics, University of Georgia, Athens, Georgia, 30602

    Mary E. Case

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  1. Mary E. Case
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Editors and Affiliations

  1. Associated Universities, Inc., Washington, D.C., USA

    Alexander Hollaender

  2. Department of Microbiology, University of Illinois, Champaign, Urbana, USA

    Ralph D. DeMoss

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© 1982 Plenum Press, New York

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Case, M.E. (1982). Transformation of Neurospora Crassa Utilizing Recombinant Plasmid DNA. In: Hollaender, A., DeMoss, R.D., Kaplan, S., Konisky, J., Savage, D., Wolfe, R.S. (eds) Genetic Engineering of Microorganisms for Chemicals. Basic Life Sciences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4142-0_10

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  • DOI: https://doi.org/10.1007/978-1-4684-4142-0_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4144-4

  • Online ISBN: 978-1-4684-4142-0

  • eBook Packages: Springer Book Archive

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