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Detection of Genetically Toxic Metals by a Microtiter Microbial DNA Repair Assay

  • Guylyn R. Warren
Part of the Environmental Science Research book series (ESRH, volume 22)

Abstract

For some years, our laboratory has been involved in assessing the genetically toxic effects of inorganic chemicals found in the environment near mining and smelting operations (Tindall et al., 1978; Warren et al., 1979) and of metal-containing pesticides used in Montana (Warren et al., 1976). Although many inorganic species are known or suspected carcinogens or are genetically active in many test systems (Flessel et al., 1979; Sunderman, 1978), most existing short-term biological screening methods are unsuitable for use with this class of suspected carcinogen. Only two systems, the Bacillus subtilus rec assay (Nishioka, 1975; Kanematsu et al., 1980) and an in vitro DNA synthesis fidelity assay (Loeb et al., 1979), have been useful for a large number of inorganic chemicals. Mutagenicity of some inorganic chemicals has been demonstrated in a CHO/HGPRT assay (Hsie et al., 1979) with considerable technical difficulty due to the general toxicity of inorganic chemicals. Green and Muriel (1976) have used a repair-deficient series of Escherichia coli B strains to detect mutagenicity of some chromate salts; also by this method, they found that nickel chloride salts do not cause differential lethality.

Keywords

Minimal Inhibitory Concentration Test Agent Ames Test Rhodium Complex Repair Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • Guylyn R. Warren
    • 1
  1. 1.Chemistry DepartmentMontana State UniversityBozemanUSA

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