Detection of Genetically Toxic Metals by a Microtiter Microbial DNA Repair Assay

  • Guylyn R. Warren
Part of the Environmental Science Research book series (ESRH, volume 22)


For some years, our laboratory has been involved in assessing the genetically toxic effects of inorganic chemicals found in the environment near mining and smelting operations (Tindall et al., 1978; Warren et al., 1979) and of metal-containing pesticides used in Montana (Warren et al., 1976). Although many inorganic species are known or suspected carcinogens or are genetically active in many test systems (Flessel et al., 1979; Sunderman, 1978), most existing short-term biological screening methods are unsuitable for use with this class of suspected carcinogen. Only two systems, the Bacillus subtilus rec assay (Nishioka, 1975; Kanematsu et al., 1980) and an in vitro DNA synthesis fidelity assay (Loeb et al., 1979), have been useful for a large number of inorganic chemicals. Mutagenicity of some inorganic chemicals has been demonstrated in a CHO/HGPRT assay (Hsie et al., 1979) with considerable technical difficulty due to the general toxicity of inorganic chemicals. Green and Muriel (1976) have used a repair-deficient series of Escherichia coli B strains to detect mutagenicity of some chromate salts; also by this method, they found that nickel chloride salts do not cause differential lethality.


Minimal Inhibitory Concentration Test Agent Ames Test Rhodium Complex Repair Assay 
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  1. Ames, B.N., J. McCann, and E. Yamasaki. 1975. Methods for detecting carcinogens and mutagens with the Salmonella/ mammalian-microsome mutagenicity test. Mutation Res. 31: 347–363.CrossRefGoogle Scholar
  2. Cooke Engineering. 1972. Handbook of Microtiter Procedures. T.B. Conrath, ed. Dynatech Corp.: Cambridge, MA. 475 pp.Google Scholar
  3. Flessel, C.P., A. Furst, and S.B. Radding. 1979. A comparison of carcinogenic metals. In: Metal Ions in Biological Systems,Volume 10, Chapter 2, Carcinogenicity and Metal Ions. H. Sigel, ed. Marcel Decker: New York.Google Scholar
  4. Green, M.H.L., and W.J. Muriel. 1976. Mutagen testing using Trp+ reversion in Escherichia coli. Mutation Res. 38: 3–32.CrossRefGoogle Scholar
  5. Greenberg, J. 1967. Loci for radiation sensitivity in Escherichia coli strain Bs_1. Genetics 55: 193–201.Google Scholar
  6. Hanawalt, P.C., P.K. Cooper, A.R. Ganesan, and G.A. Smith. 1979. DNA repair in bacteria and mammalian cells. Ann. Rev. Biochem. 48: 783–836.CrossRefGoogle Scholar
  7. Hsie, A.W., N.P. Johnson, D.B. Couch, J.R. San Sebastian, J.P. O’Neill, J.D. Hoeschele, R.O. Rahn, and N. Forbes. 1979. Quantitative mammalian cell mutagenesis and a preliminary study of the mutagenic potential of metallic compounds. In: Trace Elements in Health and Disease. N. Kharasch, ed. Raven Press: New York. pp. 55–69.Google Scholar
  8. Hyman, J., Z. Leifer, and H.S. Rosenkranz. 1980. The E. coli polA1 assay: a quantitative procedure for diffusible and non-diffusible chemicals. Mutation Res. 74: 107–111.CrossRefGoogle Scholar
  9. Kanematsu, O., M. Hara, and T. Kada. 1980. Rec assay and mutagenicity studies on metal compounds. Mutation Res. 77: 109–116.CrossRefGoogle Scholar
  10. Kimball, R.F. 1978. The relation of repair phenomena to mutation induction in bacteria. Mutation Res. 55: 85–120.CrossRefGoogle Scholar
  11. McCarroll, N.E., B.H. Keech, and C.E. Piper. 1980a. A comparative evaluation of microsuspension microbial DNA repair systems. Environ. Mutagen. 2: 270. (abstr.)Google Scholar
  12. McCarroll, N.E., C.E. Piper, G.M. Fukin, B.H. Keech, and G. Gridley. 1979. A serial dilution multiwell suspension assay for DNA damage in E. coli. Environ. Mutagen. 1: 123. (abstr.)Google Scholar
  13. McCarroll, N.E., C.E. Piper, and B.H. Keech. 1980b. Bacterial microsuspension assays with benzene and other organic solvents. Environ. Mutagen. 2: 281. (abstr.)Google Scholar
  14. Murray, M.M. 1979. Substrate specificity of uvr excision repair. Environ. Mutagen. 1: 347–352.CrossRefGoogle Scholar
  15. Nishioka, H. 1975. Mutagenic activities of metal compounds in bacteria. Mutation Res. 31: 185–189.CrossRefGoogle Scholar
  16. Radman, M. 1977. Inducible pathways in deoxyribonucleic acid repair, mutagenesis and carcinogenesis. Biochem. Soc. Trans. 5: 1194–1199.Google Scholar
  17. Sunderman, F.W.,Jr. 1978. Carcinogenic effects of metals. Fed. Proc. 37: 40–46.Google Scholar
  18. Tamaro, M., S. Venturini, C. Eftimiadi, and C. Monti-Bragadin. 1977. Interaction of platinum compounds with bacterial DNA. Experientia 33: 317–319.CrossRefGoogle Scholar
  19. Tindall, K.R. 1977. The mutagenicity of inorganic ions in microbial systems. Unpublished master’s thesis, Montana State University. University Microfilm: Bozeman, MT.Google Scholar
  20. Tindall, K.R., G.R. Warren, and P.D. Skaar. 1978. Metal ion effects in microbial screening systems. Mutation Res. 53: 9091. (abstr.)Google Scholar
  21. Tomizawa, J.-I., and H. Ogawa. 1968. Breakage of DNA in reef and rec-bacteria by disintegration of radiophosphorous atom in DNA and possible cause of pleiotropic effects of recA mutation. Cold Spring Harbor Symp. Quant. Biol. 33: 243–251.CrossRefGoogle Scholar
  22. Venturini, S., and C. Monti-Bragadin. 1978. R plasmid-mediated enhancement of mutagenesis in strains of Escherichia coli deficient in known repair functions. Mutation Res. 50: 1–8.CrossRefGoogle Scholar
  23. Warren, G., E. Abbott, P. Schultz, K. Bennett, and S. Rogers. (in press a). Mutagenicity of a series of octahedral rhodium III compounds. Mutation Res.Google Scholar
  24. Warren, G.R., S.J. Rogers, and E.H. Abbott. (in press b). The genetic toxicology of substitutionally inert transition metal complexes. In: Inorganic Chemistry in Biology and Medicine, ACS Advances in Chemistry Series.Google Scholar
  25. Warren, G., S. Rogers, S.G. Mevec, and M.D. Roach. 1979. Mutagen screening in an isolated high lung cancer mortality area of Montana. Montana Air Pollution Study, Air Quality Bureau, Dept. of Health and Environmental Sciences, Helena, MT. 30 pp.Google Scholar
  26. Warren, G., P. Schultz, D. Bancroft, K. Bennett, E.H. Abbott, and S. Rogers. (MS). Mutagenicity of a series of octahedral chromium III compounds.Google Scholar
  27. Warren, G.R., P.D. Skaar, and S.J. Rogers. 1976. Genetic activity of dithiocarbamate and thiocarbamoyl disulfide fungicides in Saccharomyces cerevisiae, Salmonella typhimurium, and Escherichia coli. Mutation Res. 38: 391–392 (abstr.).CrossRefGoogle Scholar
  28. Witkin, E.M. 1976. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol. Rev. 40: 869–907.Google Scholar
  29. Yanofsky, C. 1963. Amino acid replacements associated with mutation and recombination in the A gene and their relationship to in vitro coding data. Cold Spring Harbor Symp. Quant. Biol. 28: 581–588.CrossRefGoogle Scholar
  30. Zakour, R.A., L.A. Loeb, T.A. Kunkel, and R.M. Koplitz. 1979. Metals, DNA polymerization, and genetic miscoding. In: Trace Elements in Health and Disease. N. Kharasch, ed. Raven Press: New York. pp. 135–153.Google Scholar

Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • Guylyn R. Warren
    • 1
  1. 1.Chemistry DepartmentMontana State UniversityBozemanUSA

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