Isolation and Use of lonophores for Studying Ion Transport across Natural Membranes

  • Tom R. Herrmann
  • Adil E. Shamoo


One of the most important and interesting membrane-based processes is the transport of ions in and out of cells and cell organelles. In the study of ion transport across membranes, the concept of ionophoric activity has emerged. The term ionophoric activity has been used to mean various properties of a substance, depending on the method used to assay for the ionophorous material. Using the characteristic common to all methods, we may generally define an ionophore as a substance that enhances the movement or incorporation of an ion from an aqueous phase into a hydrophobic phase (Shamoo and Goldstein, 1977). An ionophore so defined is not necessarily involved in ion transport across biological membranes.


Sarcoplasmic Reticulum Bilayer Lipid Membrane Cyanogen Bromide Tryptic Fragment Bathing Fluid 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Tom R. Herrmann
    • 1
  • Adil E. Shamoo
    • 1
  1. 1.Department of Biological ChemistryUniversity of Maryland School of MedicineBaltimoreUSA

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