Genetic Approaches for Studying Protein Localization

  • Jeffrey L. Garwin
  • Jon Beckwith


The process of protein compartmentalization is common to both prokaryotic and eukaryotic cells. In eukaryotic cells, proteins are apportioned specifically to the cytoplasm, to any of a variety of organelles, or to the surrounding medium. While less complex, gram-negative bacteria also specifically localize proteins to subcellular compartments: cytoplasm, inner membrane, outer membrane, or periplasm (the space between the inner and the outer membrane).


Coat Protein Protein Localization Maltose Binding Protein Hybrid Protein Secretory Apparatus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Bassford, P., and Beckwith, J. (1979). Nature (London) 277, 538–541.CrossRefGoogle Scholar
  2. Bassford, P. J., Jr., Silhavy, T. J., and Beckwith, J. R. (1979). J. Bacteriol. 139, 19–31.Google Scholar
  3. Bedouelle, H., Bassford, P. J., Jr., Fowler, A. V., Zabin, I., Beckwith, J., and Hofnung, M. (1980). Nature (London) 285, 78–81.CrossRefGoogle Scholar
  4. Blobel, G., and Dobberstein, D. (1975). J. Cell Biol. 67, 835–851.CrossRefGoogle Scholar
  5. Boeke, J. D., Russel, M., and Model, P. (1980). J. Mol. Biol. 144, 103–116.CrossRefGoogle Scholar
  6. Davis, B. D., and Tai, P.-C. (1980). Nature (London) 283, 433–438.CrossRefGoogle Scholar
  7. Emr, S. D., and Silhavy, T. J. (1980). J. Mol. Biol. 141, 63–90.CrossRefGoogle Scholar
  8. Emr, S. D., Hall, M. N., and Silhavy, T. J. (1980a). J. Cell Biol. 86, 701–711.CrossRefGoogle Scholar
  9. Emr, S. D., Hedgpeth, J., Clement, J.-M., Silhavy, T. J., and Hofnung, M. (1980b). Nature (London) 285, 8285.CrossRefGoogle Scholar
  10. Emr, S. D., Hanley-Way, S., and Silhavy, T. J. (1981). Cell 23, 79–88.CrossRefGoogle Scholar
  11. Ito, K. and Beckwith, J. R. (1981). Cell 25, 143–150.CrossRefGoogle Scholar
  12. Jarvik, J., and Botstein, D. (1973). Proc. Natl. Acad. Sci. USA 70, 2046–2050.CrossRefGoogle Scholar
  13. Jarvik, J., and Botstein, D. (1975). Proc. Natl. Acad. Sci. USA 72, 2738–2742.CrossRefGoogle Scholar
  14. Jaskunas, S. R., Lindahl, L., Nomura, M., and Burgess, R. R. (1975). Nature (London) 257, 458–462.CrossRefGoogle Scholar
  15. Koshland, D., and Botstein, D. (1980). Cell 20, 749–760.CrossRefGoogle Scholar
  16. Lin, J. J. C., Kanazawa, J., Ozols, J., and Wu, H. C. (1978). Proc. Natl. Acad. Sci. USA 75, 4891–4895.CrossRefGoogle Scholar
  17. Novick, P., and Schekman, R. (1979). Proc. Natl. Acad. Sci. USA 76, 1858–1862.CrossRefGoogle Scholar
  18. Novick, P., Field, C., and Schekman, R. (1980). Cell 21, 205–215.CrossRefGoogle Scholar
  19. Oliver, D. B., and Beckwith, J. (1981). Cell 25, 765–772.CrossRefGoogle Scholar
  20. Russel, M., and Model, P. (1981). Proc. Natl. Acad. Sci. USA, 78, 1717–1721.CrossRefGoogle Scholar
  21. Sancar, A., Hack, A. M., and Rupp, W. D. (1979). J. Bacteriol. 137, 692–693.Google Scholar
  22. Silhavy, T. J., Shuman, H. A., Beckwith, J., and Schwartz, M. (1977). Proc. Natl. Acad. Sci. USA 74, 5411–5415Google Scholar
  23. Silhavy, T. J., Bassford, P. J., Jr., and Beckwith, J. R. (1979). In Bacterial Outer Membranes: Biogenesis and Functions (M. Inouye, ed.), pp. 203–254, Wiley, New York.Google Scholar
  24. Talmadge, K., Kaufman, J., and Gilbert, W. (1980a). Proc. Natl. Acad. Sci. USA 77, 3988–3992.CrossRefGoogle Scholar
  25. Talmadge, K., Stahl, S., and Gilbert, W. (1980b). Proc. Natl. Acad. Sci. USA 77, 3369–3373.CrossRefGoogle Scholar
  26. Wanner, B. L., Sarthy, A., and Beckwith, J. (1979). J. Bacteriol. 140, 229–239.Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Jeffrey L. Garwin
    • 1
  • Jon Beckwith
    • 1
  1. 1.Department of Microbiology and Molecular GeneticsHarvard Medical SchoolBostonUSA

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