Direct Demonstration of Cotranslational Secretion of Proteins in Bacteria

  • Phang-C. Tai
  • Bernard D. Davis


In animal cells a special role for the ribosomes bound to the endoplasmic reticulum was recognized early by Palade and co-workers (reviewed in Palade, 1975), who observed a parallel between the abundance of such ribosomes and the secretion of proteins. Various secretory proteins were shortly shown to be synthesized on the membrane-bound polysomes, and various cytoplasmic proteins on the free polysomes (reviewed in Rollerston, 1974). This distribution suggested that secreted proteins might cross the membrane as growing chains, rather than after completion. In support of this model, Redman and Sabatini (1966) found that puromycin released incomplete chains to the interior of the microsomes.


Signal Sequence Bacillus Licheniformis Membrane Fragment Direct Attachment Corynebacterium Diphtheriae 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Phang-C. Tai
    • 1
  • Bernard D. Davis
    • 1
  1. 1.Bacterial Physiology UnitHarvard Medical SchoolBostonUSA

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