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Synthesis and Assembly of the Vesicular Stomatitis Virus Glycoprotein and Related Glycoproteins

  • Asher Zilberstein
  • Harvey F. Lodish

Abstract

Several cell surface proteins, of quite diverse origin, have in common a number of key structural characteristics: the surface glycoproteins of vesicular stomatitis virus (VSV) and sindbis virus; influenza HA glycoprotein; and possibly surface glycoproteins of other viruses; glycophorin, a major erythrocyte surface protein; and the heavy chain of the HLAA and H-2 major histocompatibility antigens. All of these are structural proteins, and appear to be involved in cell-cell or virus-cell recognition. All of these polypeptides span the phospholipid bilayer only once (Fig. 1). In all of these proteins a region of 10–30 amino acids at the COOH terminus faces the cytoplasmic surface. This region contains a number of hydrophilic amino acids. Adjacent to this region is a sequence of 20–25 very hydrophobic amino acids believed to be the segment that spans the lipid membrane. Adjacent to either the NH2 or the COOH end of this hydrophobic stretch are often a series of lysine and arginine residues; possibly, these interact with the phosphate residues of the phospholipid bilayer. Recent work indicates that fatty acids are covalently bound to serine or threonine residues near the COOH terminus of at least some of these proteins. The remainder of the polypeptide chain (which varies considerably in size among these polypeptide species) is on the extracytoplasmic surface, as are all of the attached carbohydrate chains.

Keywords

Endoplasmic Reticulum Rough Endoplasmic Reticulum Vesicular Stomatitis Virus Phospholipid Bilayer Endoplasmic Reticulum Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Asher Zilberstein
    • 1
  • Harvey F. Lodish
    • 1
  1. 1.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA

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