Skip to main content

Photoactivated Hydrophobic Reagents for Integral Membrane Proteins

A Critical Discussion

  • Chapter
Membranes and Transport

Abstract

Photoactivated hydrophobic reagents are being developed to label those regions of integral membrane proteins that lie within the hydrocarbon core of the lipid bilayer. There are three goals. First, we wish to have a collection of reagents that react entirely from within the bilayer and to a negligible extent from the aqueous phase. Second, we would like to label, without discrimination, all the functional groups exposed to the hydrocarbon core of the membrane. A third goal is to make reagents that will label integral proteins at points that are at defined distances from the surface of the membrane.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bayley, H., and Knowles, J. R. (1978a). Biochemistry 17, 2414–2419.

    Article  CAS  Google Scholar 

  • Bayley, H., and Knowles, J. R. (1978b). Biochemistry 17, 2420–2423.

    Article  CAS  Google Scholar 

  • Bayley, H., and Knowles, J. R. (1980a). Ann. N.Y. Acad. Sci. 346, 45–58.

    Article  CAS  Google Scholar 

  • Bayley, H., and Knowles, J. R. (1980b). Biochemistry 19, 3883–3892.

    Article  CAS  Google Scholar 

  • Bercovici, T., Gitler, C., and Bromberg, A. (1978). Biochemistry 17, 1484–1489.

    Article  CAS  Google Scholar 

  • Bisson, R., and Montecucco, C. (1981). Biochem. J. 193, 757–763.

    CAS  Google Scholar 

  • Bisson, R., Montecucco, C., Gutweniger, H., and Azzi, A. (1979). J. Biol. Chem. 254 9962–9965.

    CAS  Google Scholar 

  • Brunner, J. (1981). Trends Biochem. Sci. 6, 44–46.

    Article  CAS  Google Scholar 

  • Brunner, J., and Richards, F. M. (1980). J. Biol. Chem. 255, 3319–3329.

    CAS  Google Scholar 

  • Brunner, J., and Semenza, G. (1981). Biochemistry, in press.

    Google Scholar 

  • Brunner, J., Senn, H., and Richards, F. M. (1980). J. Biol. Chem. 255, 3313–3318.

    CAS  Google Scholar 

  • Castro, J., and Farley, R. A. (1979). J. Biol. Chem. 254, 2221–2228.

    CAS  Google Scholar 

  • Cerletti, N., and Schatz, G. (1979). J. Biol. Chem. 254, 7746–7751.

    CAS  Google Scholar 

  • Chapman, O. L. (1979). Pure Appl. Chem. 51, 331–339.

    Article  CAS  Google Scholar 

  • Chowdhry, V., Vaughan, R., and Westheimer, F. H. (1976). Proc. Natl. Acad. Sci. USA 73, 1406–1408.

    Article  CAS  Google Scholar 

  • Czarnecki, M. F., and Breslow, R. (1979). J. Am. Chem. Soc. 101, 3675–3676.

    Article  Google Scholar 

  • Engelman, D. M., Henderson, R., McLachlan, A. D., and Wallace, B. A. (1980). Proc. Natl. Acad. Sci. USA 77, 2023–2027.

    Article  CAS  Google Scholar 

  • Erni, B., and Khorana, H. G. (1980). J. Am. Chem. Soc. 102, 3888–3896.

    Article  CAS  Google Scholar 

  • Farley, R. A., Goldman, D. W., and Bayley, H. (1980). J. Biol. Chem. 255, 860–864.

    CAS  Google Scholar 

  • Frank, G., Brunner, J., Hauser, H., Wacker, H., Semenza, G., and Zuber, H. (1978). FEBS Lett. 96 183–188.

    Article  CAS  Google Scholar 

  • Galardy, R. E., Craig, L. C., and Printz, M. P. (1973). Nature New Biol. 242, 127–128.

    CAS  Google Scholar 

  • Girdlestone, J., Bisson, R., and Capaldi, R. A. (1981). Biochemistry 20, 152–156.

    Article  CAS  Google Scholar 

  • Goldman, D. W., Pober, J. S., White, J., and Bayley, H. (1979). Nature (London) 280 841–843.

    Article  CAS  Google Scholar 

  • Gupta, C. M., Costello, C. E., and Khorana, H. G. (1979). Proc. Natl. Acad. Sci. USA 76 3139–3143.

    Article  CAS  Google Scholar 

  • Hu, V. W., and Wisnieski, B. J. (1979). Proc. Natl. Acad. Sci. USA 76 5460–5464.

    Article  CAS  Google Scholar 

  • Hu, V. W., Esser, A. F., Podack, E. R., and Wisnieski, B. (1981). J. Immunol., in press.

    Google Scholar 

  • Jorgenson, P. L., Karlish, S. J. D., and Gitter, C. (1982). J. Biol. Chem.,in press.

    Google Scholar 

  • Kahane, I., and Gitler, C. (1978). Science 201 351–352.

    Article  CAS  Google Scholar 

  • Karfish, S. J. D., Jorgensen, P. L., and Gitler, C. (1977). Nature (London) 269 715–717.

    Article  Google Scholar 

  • Kaufman, J. F., and Strominger, J. L. (1979). Proc. Natl. Acad. Sci. USA 76 6304–6308.

    Article  CAS  Google Scholar 

  • Kerr, J. A., O’Grady, B. V., and Trotman-Dickenson, A. F. (1967). J. Chem. Soc. A, 897.

    Google Scholar 

  • Mas, M. T., Wang, J. K., and Hargrave, P. A. (1980). Biochemistry 19 684–692.

    Article  CAS  Google Scholar 

  • Montecucco, C., Bisson, R., Gache, C., and Johannsson, A. (1981). FEBS Let. 128 17–21.

    Article  CAS  Google Scholar 

  • Nielsen, P. E., Leick, V., and Buchardt, O. (1978). FEBS Lett. 94 287–290.

    Google Scholar 

  • Owen, M. J., Knott, J. C. A., and Crumpton, M. J. (1980). Biochemistry 19 3092–3099.

    Article  CAS  Google Scholar 

  • Ploegh, H. L., On, H. T., and Strominger, J. L. (1980). Proc. Natl. Acad. Sci. USA 77 6081–6085.

    Article  CAS  Google Scholar 

  • Prochaska, L., Bisson, R., and Capaldi, R. A. (1980). Biochemistry 19 3174–3177.

    Article  CAS  Google Scholar 

  • Quay, S. C., Radhakrishnan, R., and Khorana, H. G. (1981). J. Biol. Chem. 256 4444–4449.

    CAS  Google Scholar 

  • Radhakrishnan, R., Gupta, C. M., Erni, B., Robson, R. J., Curatolo, W., Majumdar, A., Ross, A. H., Takagaki, Y., and Khorana, H. G. (1980). Ann. N.Y. Acad. Sci. 346 165–198.

    Article  CAS  Google Scholar 

  • Radhakrishnan, R., Robson, R. J., Takagaki, Y., and Khorana, H. G. (1981). Methods Enzymol., 72 408–433.

    Article  CAS  Google Scholar 

  • Robson, R. J., Radhakrishnan, R., Ross, A. H., Takagaki, Y., and Khorana, H. G. (1981). In Lipid-Protein Interactions (O. H. Griffiths and P. Jost, eds.), Wiley, New York, in press.

    Google Scholar 

  • Ross, A. H., and Khorana, H. G. (1982). Submitted for publication.

    Google Scholar 

  • Sator, V., Gonzalez-Ros, J. M., Calvo-Fernandez, P., and Martinez-Carrion, M. (1979). Biochemistry 18 1200–1206.

    Article  CAS  Google Scholar 

  • Simon, P., and Wisnieski, B. J. (1980). Fed. Proc. 39 2190.

    Google Scholar 

  • Smith, R. A. G., and Knowles, J. R. (1975). J. Chem. Soc. Perkin Trans. 2, 686–694.

    Google Scholar 

  • Staros, J. V. (1980). Trends Biochem. Sci. 5 320–322.

    Article  CAS  Google Scholar 

  • Stoffel, W., Schreiber, C., and Scheefers, H. (1978). Hoppe-Seyler’s Z. Physiol. Chem. 359 923–931.

    Article  CAS  Google Scholar 

  • Tarrab-Hazdai, R., Bercovici, T., Goldfarb, V., and Gitler, C. (1980). J. Biol. Chem. 255 1204–1209.

    CAS  Google Scholar 

  • Tomasi, M., and Montecucco, C. (1981). J. Biol. Chem. 256 11177–11181.

    CAS  Google Scholar 

  • Wells, E., and Findlay, J. B. C. (1980). Biochem. J. 187 719–725.

    CAS  Google Scholar 

  • Wisnieski, B. J., and Bramhall, J. S. (1981). Nature (London) 289 319–323.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1982 Plenum Press, New York

About this chapter

Cite this chapter

Bayley, H. (1982). Photoactivated Hydrophobic Reagents for Integral Membrane Proteins. In: Martonosi, A.N. (eds) Membranes and Transport. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4082-9_24

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-4082-9_24

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4084-3

  • Online ISBN: 978-1-4684-4082-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics