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Saturation Transfer EPR Studies of Rotational Dynamics in Membranes

  • David D. Thomas

Abstract

Beginning about 10 years ago, electron paramagnetic resonance (EPR) studies on spinlabeled lipids, pioneered by McConnell and co-workers, have helped elucidate the dynamic properties of the lipid components of both synthetic and biological membranes. While the conventional EPR technique is ideally suited to monitor the nanosecond rotational motions of fluid lipid hydrocarbon chains or small protein segments, that technique is insensitive to the slower motions (often in the microsecond range) that are typical for gel-phase lipid and entire membrane proteins. In contrast, saturation transfer EPR (ST-EPR), a technique introduced by Hyde and Dalton (1972), has its optimum sensitivity in the microsecond region (recent reviews: Thomas, 1978; Hyde and Dalton, 1979; Hyde and Thomas, 1980). Since the introduction of the ST-EPR capability in commercial spectrometers about 4 years ago, this technique has proven to be a powerful tool in the study of membrane dynamics, particularly the rotational motions of integral membrane proteins.

Keywords

Electron Paramagnetic Resonance Spin Label Integral Membrane Protein Rotational Dynamics Nitroxide Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baroin, A., Thomas, D. D., Osborne, R., and Devaux, P. F. (1977). Biochem. Biophys. Res. Commun. 78, 442–449.CrossRefGoogle Scholar
  2. Baroin, A., Bienvenüe, A., and Devaux, P. F. (1979). Biochemistry 18, 1151–1155.CrossRefGoogle Scholar
  3. Davoust, J., Bienvenüe, A., Fellmann, P., and Devaux, P. F. (1980). Biochim. Biophys. Acta 596, 28–42.CrossRefGoogle Scholar
  4. Delmelle, M., Butler, K. W., and Smith, I. C. P. (1980). Biochemistry 19, 698–704.CrossRefGoogle Scholar
  5. Devaux, P. F., Baroin, A., Bienvenüe, A., Favre, E., Rousselet, A., and Thomas, D. D. (1978). In Bioenergetics of Membranes ( L. Packer, ed.), pp. 47–54, Academic Press, New York.Google Scholar
  6. Favre, E., Baroin, A., Bienvenüe, A., and Devaux, P. F. (1979). Biochemistry 18, 1156–1162.CrossRefGoogle Scholar
  7. Fung, L. W.-M., Soo Hoo, M. J., and Meena, W. A. (1979). FEBS Lett. 105, 379–383.CrossRefGoogle Scholar
  8. Hidalgo, C., Thomas, D. D., and Ikemoto, N. (1978). J. Biol. Chem. 253, 6879–6887.Google Scholar
  9. Hyde, J. S., and Dalton, L. R. (1972). Chem. Phys. Lett. 16, 568–572.CrossRefGoogle Scholar
  10. Hyde, J. S., and Dalton, L. R. (1979). In Spin Labeling II ( L. Berlina, ed.), pp. 1–70, Academic Press, New York.Google Scholar
  11. Hyde, J. S., and Thomas, D. D. (1980). Annu. Rev. Phys. Chem. 31, 293–317.CrossRefGoogle Scholar
  12. Kaizu, T., Kirino, Y., and Shimizu, H. (1980). J. Biochem. (Tokyo) 88, 1837–1843.Google Scholar
  13. Kirino, Y., Ohkuma, T., and Shimizu, H. (1978). J. Biochem. (Tokyo) 84, 111–115.Google Scholar
  14. Kusumi, A., Ohnishi, S., Ito, T., and Yoshizawa, T. (1978). Biochim. Biophys. Acta 507, 539–543.CrossRefGoogle Scholar
  15. Kusimi, A., Sakaki, T., Yoshizawa, T., and Ohnishi, S. (1980). J. Biochem. (Tokyo) 88, 1103–1111.Google Scholar
  16. Marsh, D. (1980). Biochemistry 19, 1632–1637.CrossRefGoogle Scholar
  17. Robinson, B. H., and Dalton, L. R. (1980). J. Chem. Phys. 72, 1312–1324.CrossRefGoogle Scholar
  18. Rousselet, A., and Devaux, P. (1977). Biochem. Biophys. Res. Commun. 78, 448–454.CrossRefGoogle Scholar
  19. Swanson, M. S., Quintanilha, A. T., and Thomas, D. D. (1980). J. Biol. Chem. 255, 7494–7502.Google Scholar
  20. Swift, L. L., Atkinson, J. B., Perkins, R. C., Jr., Dalton, L. R., and Le Quire, V. S. (1980). J. Membr. Biol. 52, 165–172.CrossRefGoogle Scholar
  21. Thomas, D. D. (1978). Biophys. J. 24, 211–223.CrossRefGoogle Scholar
  22. Thomas, D. D., and Hidalgo, C. (1978). Proc. Natl. Acad. Sci. USA 75, 5488–5492.CrossRefGoogle Scholar
  23. Thomas, D. D., Dalton, L. R., and Hyde, J. S. (1976). J. Chem. Phys. 65, 3006–3024.CrossRefGoogle Scholar
  24. Wilkerson, L. S., Perkins, R. C., Jr., Roelofs, R., Swift, L., Dalton, L. R., and Park, J. H. (1978). Proc. Natl. Acad. Sci. USA 75, 838–841.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • David D. Thomas
    • 1
  1. 1.Department of BiochemistryUniversity of Minnesota Medical SchoolMinneapolisUSA

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