NMR in the Study of Membranes
The use of NMR for the study of membranes was introduced by Chapman and Salsbury (1966). Over the past decade several hundred reports of observations of 1H, 2H, 13C, 19F, and 31P spectra on phospholipid vesicles and several natural membranes have appeared. The majority of these have addressed various aspects of the problems of order, orientation, mobility, and lateral diffusion in phospholipid bilayers and multilayers. Others have dealt with the conformations of the polar head groups of phospholipids, transmembrane exchange (flip-flop) between the two layers, interactions of small molecules—notably cholesterol—and local anesthetics with both model and natural membranes, and function of ion channels in reconstituted membranes, the fusion of phospholipid bilayers catalyzed by peptides and proteins, and the nature of protein-lipid interactions.
KeywordsSpin Label Lateral Diffusion Phospholipid Bilayer Polar Head Group Phospholipid Vesicle
Unable to display preview. Download preview PDF.
- Brown, T. R., Ugurbil, K., and Shulman, R. G. (1977). Proc. Natl. Acad. Sci. USA 74, 551–553.Google Scholar
- Chapman, D. (1968). In Biological Membranes(D. Chapman, ed.), pp. 189–202, Academic Press, New York.Google Scholar
- Finer, E. G., Henry, R., Leslie, R. B., and Robertson, R. S. (1975). Biochim. Biophys. Acta 380, 320–337.Google Scholar
- Hamilton, J. A., and Cordes, E. H. (1978). J. Biol. Chem 253, 5193–5198.Google Scholar
- Jardetzky, O. (1980). Biochim. Biophys. Acta 621, 227–232.Google Scholar
- Jardetzky, O., and Roberts, G. C. K. (1981). NMR in Molecular Biology, Academic Press, New York.Google Scholar
- Lee, A. G., Birdsall, N. J. M., Levine, Y. K., and Metcalfe, J. C. (1972). Biochim. Biophys. Acta 225, 43–56.Google Scholar
- Luzzati, V. (1968). In Biological Membranes(D. Chapman, ed.), pp. 71–123, Academic Press, New York.Google Scholar
- Seelig, J. (1978). Biochim. Biophys. Acta 515, 105–140.Google Scholar