Electron Microscopy and Computer Image Reconstruction of Membrane Crystals

  • K. R. Leonard
  • H. Weiss


Membrane proteins exist partially in a phospholipid bilayer and partially in the adjacent aqueous medium. For structural stability they therefore need as their environment a narrow band of nonpolar hydrocarbon bounded by polar groups. A pure aqueous solution is not satisfactory; likewise, a simple organic solvent would be unsuitable. The native environment can, however, be simulated successfully by detergent micelles (Tanford and Reynolds, 1976; Helenius et al., 1979). This partially hydrophobic and partially hydrophilic property of membrane proteins renders it difficult to prepare three-dimensional crystals that are suitable for high-resolution single-crystal X-ray analysis. So far, only a small number of membrane proteins have been reported to crystallize (Tanaka et al.,1980; Michel and Oesterhelt, 1980; Garavito and Rosenbusch, 1980).


Phospholipid Bilayer Purple Membrane Dimensional Reconstruction Beef Heart Detergent Micelle 


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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • K. R. Leonard
    • 1
  • H. Weiss
    • 1
  1. 1.European Molecular Biology LaboratoryHeidelbergWest Germany

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