Immunochemical Analysis of the Antigenic Profile of Human Melanoma Cells with Monoclonal Antibodies

  • Barry S. Wilson
  • Kohzoh Imai
  • Pier-Giorgio Natali
  • Neil E. Kay
  • Renato Cavaliere
  • Michele A. Pellegrino
  • Soldano Ferrone


Tumor immunologists have been investigating the antigenic profile of cancer cells in order to define cellular changes induced by malignant transformation. Characterization of these changes may aid our understanding of the interaction between tumor cells and the host’s immune system, may identify markers to determine the molecular basis of malignant transformation, and may provide unique antigenic determinants to apply immunological approaches for diagnosis and therapy of tumors. We have focused our efforts on melanoma, since this tumor represents a type of human cancer in which the immune response is believed to participate in its pathogenicity and may affect its clinical course. This belief is supported by clinical evidence and by the detection of cellular and humoral immunity to tumor-associated antigens in melanoma patients. Interested readers may refer to recent reviews on these topics (Hellström and Hellström, 1974; Ferrone and Pellegrino, 1978, 1979; Mastrangelo et al., 1979). We have investigated the expression of cytoplasmic and plasma-membrane-bound tumor-associated antigens as well as of histocompatibility antigens on human melanoma cells. The latter antigens have been included in our studies because of their role as informational molecules in the immune response (Benacerraf and McDevitt, 1972) and because of their functional and structural relationships with tumor-associated antigens in certain tumor systems (Callahan et al., 1978; Curry et al., 1979).


Melanoma Cell Melanoma Cell Line Antigenic Determinant Thymic Carcinoma Histocompatibility Antigen 


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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Barry S. Wilson
    • 1
  • Kohzoh Imai
    • 1
  • Pier-Giorgio Natali
    • 2
  • Neil E. Kay
    • 1
  • Renato Cavaliere
    • 2
  • Michele A. Pellegrino
    • 1
  • Soldano Ferrone
    • 1
  1. 1.Department of Molecular ImmunologyScripps Clinic and Research FoundationLa JollaUSA
  2. 2.Istituto Regina ElenaRomeItaly

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