Cellular and Humoral Studies of Malignant Melanoma

  • Ariel Hollinshead
  • Keith Tanner
  • W. Daniel Kundin


Recent studies have provided additional information with regard to antigenic properties of melanoma cells. Some of these studies have been performed using material shed into culture media during the growth of melanoma cells. Reisfeld et al.(1980) describe melanoma-associated antigens (MAAs) shed in the culture media of radiolabeled melanoma cells, which react with polyclonal antimelanoma xenoantisera. MGP-1, a subunit of a larger structure, was described an antigen with a molecular weight of 240,000 that was exclusive to melanoma cells, and MGP-2, a single-chain antigen with a molecular weight of 94,000, was found to be present on melanoma cells, carcinoma cells, and fetal melanocytes. It was found that radiochemically pure antigens had different charge properties. In a recent review, these authors described the many variables to be considered in the evaluation of MAAs (Ferrone and Pellegrino, 1979). Gupta et al.(1979a) studied the antigenic activity of spent serum-free, chemically defined media by monitoring complement fixation and immune adherence. This group described two distinct antigens, namely, oncofetal antigen and tumor-associated antigen, (TAA). In a further study (Gupta and Morton, 1980), this group described the same TAA, derived from the M14 melanoma cell line after radiolabeling with 125I in radioimmunoassays (RIAs) with patient sera. Positive antibodies (positive antibody was defined as ≥ 1: 100 serum dilution) were in 65% of melanoma sera, 36% of carcinoma sera, and 15% of normal sera. Spent media of melanoma cell lines, or tumor extracts (63% of both), gave 50% inhibition, but little or no inhibition was seen using the spent media of sarcoma (0%), colon or breast cell lines (17%), or extracts of fetal and normal tissues (0%).


Melanoma Cell Melanoma Patient Melanoma Cell Line Primary Melanoma Bacillus Calmette Guerin 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Ariel Hollinshead
    • 1
  • Keith Tanner
    • 1
  • W. Daniel Kundin
    • 1
  1. 1.Division of Hematology and Oncology, Department of MedicineThe George Washington University Medical CenterUSA

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