Photon-Correlation Spectroscopy of Centrifuged Solutions. Application to Artemia Ribosome Subunits
Quasi-elastic light scattering can be used to probe the dynamics of macromolecules in solution (see the review by Bloomfield and Lim, 1978, and papers in this volume). In our laboratory, photon-count autocorrelation spectroscopy of scattered laser light is mostly used to measure the translational diffusion coefficient of biological macromolecules as a part of their physical-chemical characterization. In particular, we have studied the ribosomes isolated from an eukary-ote, the brine shrimp Artemia (Nieuwenhuysen and Clauwaert, 1978). An extension of this work to the ribosomal subunits is hampered by their high tendency to aggregate. The aggregation was first avoided as far as possible, then photon-correlation spectroscopy was performed on the monomeric subunits directly after they were separated from their eventual aggregates by sedimentation in a transparent centric fugation tube.
KeywordsRibosome Subunit Brine Shrimp Translational Diffusion Coefficient Monomeric Subunit Elastic Light Scattering
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- P. Nieuwenhuysen and J. Clauwaert, in The Brine Shrimp Artemia, Vol. 2., Physiology, Biochemistry, Molecular Biology, ed. by G. Persoone, P. Sorgeloos, O. Roels, and E. Jaspers., Universa Press, Wetteren, Belgium (1980), p. 491.Google Scholar