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Photon-Correlation Spectroscopy of Centrifuged Solutions. Application to Artemia Ribosome Subunits

  • Paul Nieuwenhuysen
  • Julius Clauwaert
Part of the NATO Advanced Study Institutes Series book series (NSSB, volume 73)

Abstract

Quasi-elastic light scattering can be used to probe the dynamics of macromolecules in solution (see the review by Bloomfield and Lim, 1978, and papers in this volume). In our laboratory, photon-count autocorrelation spectroscopy of scattered laser light is mostly used to measure the translational diffusion coefficient of biological macromolecules as a part of their physical-chemical characterization. In particular, we have studied the ribosomes isolated from an eukary-ote, the brine shrimp Artemia (Nieuwenhuysen and Clauwaert, 1978). An extension of this work to the ribosomal subunits is hampered by their high tendency to aggregate. The aggregation was first avoided as far as possible, then photon-correlation spectroscopy was performed on the monomeric subunits directly after they were separated from their eventual aggregates by sedimentation in a transparent centric fugation tube.

Keywords

Ribosome Subunit Brine Shrimp Translational Diffusion Coefficient Monomeric Subunit Elastic Light Scattering 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Paul Nieuwenhuysen
    • 1
  • Julius Clauwaert
    • 1
  1. 1.Department of Cell BiologyUniversity of AntwerpWilrijkBelgium

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