Quasielastic Light Scattering Studies of Microtubule Assembly in Vitro

  • G. R. Palmer
  • D. B. Sattelle
Part of the NATO Advanced Study Institutes Series book series (NSSB, volume 73)


Quasi-elastic light scattering (QELS) is a rapid, non-invasive technique for the measurement of macromolecular hydrodynamic radii and size distribution (polydispersity).1,2 This method has recently been applied to the study of the self-assembly of supramolecular structures. 3,4,5 Its usefulness in studying such heterogeneous systems is based on:
  1. (a)

    the capacity to probe the system at equilibrium in a non-perturbative manner;

  2. (b)

    a very high sensitivity to the appearance of polymeric forms in the presence of high concentrations of monomer;

  3. (c)

    the ability to obtain quantitative data without prior physical separation of the components.



Microtubule Assembly Tubulin Dimer Brain Tubulin Beef Brain Hydrodynamic Particle Size 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • G. R. Palmer
    • 1
  • D. B. Sattelle
    • 1
  1. 1.Department of ZoologyA. R. C. UnitCambridgeUK

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