Heat Aggregation of Human IgG A Dynamic Light Scattering Study
The thermal stability of immunoglobins is a critical parameter both to experimental immunologists and clinicians responsible for administration of antibody preparations. In diagnostic immunology heating of serum at 56C for 30 minutes has become a well established method of inactivating complement in immunological assays. This procedure usually does not influence the antibody activity or the other main biological properties of the immunoglobins. In contrast, heating of IgG at 63°C for 30 minutes is a widely used method to produce soluble IgG aggregates. Such aggregates possess many biological properties which make them suitable for use as controls in studies concerning soluble antigen-antibody complexes. 1,2 The structure of the heat aggregates and the mechanism by which they are formed has been studied by several methods. For the analysis of the hydrodynamic properties, ultracentrifugation and &elfiltration have been the most used techniques,3,4,5 and for the study of the biological properties of the soluble aggregates, complement fixation methods has been shown to be very sensitive.6 By dynamic light scattering we measure the diffusion coefficients of the components in a solution of IgG. In this way we can follow the process of aggregation under different conditions.
KeywordsHeat Aggregation Initial Growth Rate Soluble Aggregate Monomer Fraction Horse Spleen Ferritin
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