Phase Separation of Cytoplasma in the Lens

  • Toyoichi Tanaka
  • Izumi Nishio
  • Shao-Tang Sun
Part of the NATO Advanced Study Institutes Series book series (NSSB, volume 73)

Abstract

We would like to present our study on the phase separation of cytoplasma in the animal lens with application to cataract diseases. The. following subjects will be discussed: 1) The protein-water mixture does undergo phase separation and shows critical phenomena; 2) the cold cataract, which is the opacification of an excised lens of a young vertebrate upon lowering the temperature, is a manifestation of the phase separation of the lens cytoplasma; 3) the nuclear opacity, which appears in the lens of a galactosemic rat is due to such phase separation; and 4) the diffusivity of protein molecules in the lens cytoplasma can be determined in vivo using the technique of laser light scattering spectroscopy. The protein diffusivity is a direct measure of the susceptibility of the lens to the phase separation.

Keywords

Entropy Enthalpy Retina Dehydration Peri 

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References

  1. 1.
    G. B. Benedek, Applied Optics 10: 459 (1971).ADSCrossRefGoogle Scholar
  2. 2.
    J. H. Kinoshita, L. O. Meorla, and E. Dikmak, Exp. Eye Res. 1: 405 (1962).CrossRefGoogle Scholar
  3. 3.
    A. Spector, S. Li, and J. Sigelman, Invest. Ophthalmol. 13: 795 (1974).Google Scholar
  4. 4.
    J. A. Jedzniak, J. H. Kinoshita, E. M. Yate, and G. B. Benedek, Exp. Eye Res. 20: 367 (1975).CrossRefGoogle Scholar
  5. 5.
    C. Ishimoto and T. Tanaka, Phys. Rev. Lett. 39: 474 (1977).ADSCrossRefGoogle Scholar
  6. 6.
    T. Tanaka, C. Ishimoto, and L. T. Chylack, Jr., Science 197: 1010 (1977).ADSCrossRefGoogle Scholar
  7. 7.
    C. Ishimoto, P. W. Goalwin, S.-T. Sun, I. Nishio, and T. Tanaka, Proc. Natl. Acad. Sci. U.S.A. 76: 4414 (1979).ADSCrossRefGoogle Scholar
  8. 8.
    T. Tanaka and C. Ishimoto, Invest. Ophthalmol. 16: 135 (1977).Google Scholar
  9. 9.
    R. E. Dickerson and I. Geis, The Structure and Action of Proteins, (Harper and Row, New York, 1969).Google Scholar
  10. 10.
    C. Tanford and M. L. Wagner, J. Am. Chem. Soc. 76: 3331 (1954).CrossRefGoogle Scholar
  11. 11.
    D. Nicoli and G. B. Benedek, Biopolymers 15: 2421 (1976).CrossRefGoogle Scholar
  12. 12.
    L. D. Landau and E. M. Lifshitz, Statistical Physics, (Pergamon, Oxford, England, 1969), 2nd rev. English ed.Google Scholar
  13. 13.
    K. Kawasaki, in Phase Transitions and Critical Phenomena, edited by C. Domb and M. S. Green, (Academic, London, 1976), vol. 5A.Google Scholar
  14. 14.
    T. Tanaka, Phys. Rev. A17: 763 (1978).ADSGoogle Scholar
  15. 15.
    R. W. Young and H. W. Fulhrorst, Invest. Ophthalmol. 5: 288 (1966).Google Scholar
  16. 16.
    B. Philipson, Invest. Ophthalmol. 8: 258 (1969).Google Scholar
  17. 17.
    J. I. Clark and G. B. Benedek, Biochem. and Biophys. Res. Comm. 96: 482 (1980).CrossRefGoogle Scholar
  18. 18.
    S. Lerman and S. Zigman, Acta Ophthalmol. 45: 193 (1967).Google Scholar
  19. 19.
    R. Van Heyningen, Exp. Eye Res. 11: 415 (1971).CrossRefGoogle Scholar
  20. 20.
    T. O. Sipple, Invest. Ophthalmol. 5: 568 (1966).Google Scholar
  21. 21.
    J. H. Kinoshita, Invest. Ophthalmol. 13: 713 (1971).Google Scholar
  22. 22.
    B. Philipson, Invest. Ophthalmol. 8: 281 (1969).Google Scholar
  23. 23.
    G. B. Benedek, J. I. Clark, E. Serrallach, C. Y. Young, L. Mengel, T. Sauke, A. Bagg, and K. Benedek, Phil. Trans. Roc. Soc. London 292: 121 (1979).Google Scholar
  24. 24.
    V. E. Kinsey and K. R. Hightower, Exp. Eye Res. 26: 521 (1978).CrossRefGoogle Scholar
  25. 25.
    AMERICAN NATIONAL STANDARD Z136.1–1976.Google Scholar
  26. 26.
    O. Pomerantzeff, J. Govignon, and C. L. Schepens, Trans. Am. Acad. Ophthalmol. Otolaryngol. 73: 246 (1969).Google Scholar
  27. 27.
    R. A. Weale, Optical Acta 1: 107 (1954).ADSCrossRefGoogle Scholar
  28. 28.
    D. E. Koppel, J. Chem. Phys. 57: 4818 (1972).ADSCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Toyoichi Tanaka
    • 1
  • Izumi Nishio
    • 1
  • Shao-Tang Sun
    • 1
  1. 1.Department of Physics and Center for Materials Science and EngineeringMassachusetts Institute of TechnologyCambridgeUSA

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