Light Scattering Study of Ganglioside Micelles and Mixed Micelles with a Nonionic Amphiphile
Aqueous solutions containing a mixture of two amphiphiles, a commercial nonionic surfactant, Triton X-100, and a biological lipid, the ganglioside GM1, are investigated by static and dynamic light scattering. It is found that mixed micelles are formed in the whole range of investigated molar ratios. The theory of light scattering from solutions of homogeneous micelles is generalized to the case of mixed micelles. The final formula is used to derive from the experimental data the aggregation number of the mixed micelle. A simple phenomenological law is proposed to describe the dependence of the aggregation number on the molar ratio between the two amphiphiles. The biochemical implications of the obtained results are shortly discussed.
KeywordsMixed Micelle Aggregation Number Disodium EDTA Refractive Index Increment Free Monomer
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