Advertisement

The Shapes of the Radiation Dose-Mutation Response Curves in Drosophila: Mechanisms and Implications

  • S. Abrahamson
  • H. U. Meyer
  • C. DeJongh
Part of the Environmental Science Research book series (ESRH, volume 21)

Abstract

The major thesis we wish to make in this presentation is that radiation induced mutations, namely sex-linked recessive lethals in Drosophila and forward mutations at specific loci in Drosophila, mammals and lower eucaryotes, are the result of two sub-lesions or hits, induced by either single ionization tracks or by the interaction of two independent tracks for low LET radiations, when the dose is delivered in an acute fashion. This statement formally reduces to the well recognized linear quadratic expression: Y = C + αD + βD2 where C is the spontaneous frequency of events scored and α and β represent the coefficients of the dose. In practice, the curve is more complex because saturation occurs at high doses. This linear-quadratic relationship is most often applied to the yield of gross chromosome aberrations in plant and animal systems and was first demonstrated by Sax (1940). Lea (1955) and subsequently Neary (1965) and Kellerer and Rossi (1972) have developed the biophysical and microdosimetric framework for the empirically derived dose-response relationship.

Keywords

Dose Rate Chromosome Aberration Neutron Energy Mouse Oocyte Acute Dose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abrahamson, S., 1976, Mutation process at low or high radiation doses, in: “Biological and Environmental Effects of Low-Level Radiation, Vol. I, IAEA/STI/PUB/409 (International Atomic Energy Agency, Vienna).Google Scholar
  2. Abrahamson, S., Bender, M.A., Conger, A.D., and Wolff, S., 1973, Uniformity of radiation-induced mutation rates among different species, Nature, 245: 460.PubMedCrossRefGoogle Scholar
  3. Abrahamson, S., and Meyer, H.U., 1976, Quadratic analysis for the induction of recessive lethal mutations in Drosophila oogonia by X-irradiation, Vol. I, IAEA/STI/PUB/409 (International Atomic Energy Agency, Vienna).Google Scholar
  4. Abrahamson, S., and Wolff, S., 1976, Reanalysis of radiation induced specific locus mutations in the mouse, Nature, 264: 715.PubMedCrossRefGoogle Scholar
  5. Brewen, J.G., 1977, The application of mammalian cytogenetics to mutagenicity studies, in: “Proc. II Int. Conf. on Environ. Mutagens, Progress in Genetic Toxicology”, D. Scott, B.A. Bridges, F.H. Sobels, eds., Elsevier, North Holland.Google Scholar
  6. Brewen, J.G., and Payne, H.S., 1979, X-ray stage sensitivity of mouse oocytes and its bearing on dose-response curves, Genetics, 91: 149.PubMedGoogle Scholar
  7. Brewen, J.G., Preston, R.J., and Littlefield, L.G., 1972, Radiation-induced human chromosome aberration yields following an accidental whole-body exposure to 60Co γ rays, Radiat. Res., 49: 647.PubMedCrossRefGoogle Scholar
  8. Brewen, J.G., Payne, H.S., and Preston, R.J., 1976, X-ray induced chromosome aberrations in mouse dictyate oocytes. I. Time and dose relationship, Mutat. Res., 35: 111.PubMedCrossRefGoogle Scholar
  9. Brewen, J.G., Payne, H.S., and Adler, J.D., 1977, X-ray induced chromosome aberrations in mouse dictyate oocytes. II. Fractionation and dose rate effects, Genetics, 87: 699.PubMedGoogle Scholar
  10. Gonzalez, F.W., 1972, Dose response kinetics of genetic effects induced by 250 kVp x rays and 0.68 MeV neutrons in mature sperm of Drosophila melanogaster, Mutat. Res., 15: 303.PubMedCrossRefGoogle Scholar
  11. Herskowitz, I.H., and Abrahamson, S., 1955, The effect of X-ray intensity on the rate of sex-linked recessive lethal mutations induced by treatment of Drosophila oocytes, Drosophila Information Serv., 29: 125.Google Scholar
  12. ICRP, 1972, International Commission on Radiological Protection, The RBE of high-LET radiations with respect to mutagenesis, ICRP Publication 18, Pergamon Press, New York.Google Scholar
  13. Kellerer, A.M., and Rossi, H.H., 1972, The theory of dual radiation action, Curr. Topics Radiat. Res. Quart., 8: 85.Google Scholar
  14. Lea, D.E., 1955, “Actions of Radiations on Living Cells”, The University Press, Cambridge.Google Scholar
  15. Lyon, M.F., Morris, J., Glenister, P., and O’Grady, S.E., 1970, Induction of translocations in mouse spermatogonia by X-ray doses divided into many small fractions, Mutat. Res., 9: 219.PubMedCrossRefGoogle Scholar
  16. Lyon, M.F., Phillips, R.J.S., and Fisher, G., 1979, Dose-response curves for radiation-induced mutations in mouse oocytes and their interpretation, Mutat. Res., 63: 161.PubMedCrossRefGoogle Scholar
  17. Markowitz, E.H., 1970, Gamma ray-induced mutations in Drosophila melanogaster oocytes: The phenomenon of dose rate, Genetics, 64: 313.PubMedGoogle Scholar
  18. Meyer, H.U., and Abrahamson, S., 1978, Linear-quadratic dose kinetics for X-ray induced recessive mutations in Drosophila oogonia, Mutat. Res. 53: 229.Google Scholar
  19. Muller, H.J., Oster, I.I., and Zimmering, S., 1963, Are chronic and acute gamma irradiation equally mutagenic in Drosophila?, in: “Repair from Genetic Radiation Damage”, F.H. Sobels, ed., Pergamon Press, Oxford.Google Scholar
  20. NCRP, 1980, National Council on Radiation Protection and Measurements, Influence of dose and its distribution in time on dose-response relationships for low-LET radiations, NCRP Report No. 64, Washington, D.C.Google Scholar
  21. Neary, G.H., 1965, Chromosome aberrations and the theory of RBE. I. General Considerations, Int. J. Rad. Biol. 9: 477.CrossRefGoogle Scholar
  22. Oftedal, P., 1964a, Radiosensitivity of Drosophila spermatogonia. I. Acute doses, Genetics 49: 181.PubMedGoogle Scholar
  23. Oftedal, P., 1964b, Radiosensitivity of Drosophila spermatogonia. II. Protracted doses, Hereditas, 51: 13.CrossRefGoogle Scholar
  24. Parker, D.R., 1963, On the nature of sensitivity changes in oocytes of Drosophila melanogaster, in: Repair from Genetic Radiation Damage, F.H. Sobels, ed., Pergamon Press, Oxford.Google Scholar
  25. Pomerantzeva, M.D., Ramaiya, L.K., and Ivanov, V.N., 1972, The mutagenic effect of different types of irradiation on the germ cells of male mice. VII. The effect of dose-rate on gamma-irradiation on the induction of reciprocal translocations in spermatogonia, Genetika, 8: 128.Google Scholar
  26. Preston, R.J., and Brewen, J.G., 1973, X-ray induced translocation in spermatogonia. I. Dose and fractionation responses in mice, Mutat. Res. 19: 215.PubMedCrossRefGoogle Scholar
  27. Preston, R.J. and Brewen, J.G., 1976, X-ray induced translocations in spermatogonia. II. Fractionation responses in mice, Mutat. Res., 36: 333.PubMedCrossRefGoogle Scholar
  28. Purdom, C.E., 1963, the effect of intensity and fractionation on radiation-induced mutation in Drosophila, in: “Repair from Genetic Radiation Damage”, F.H. Sobels, ed., MacMillan Press, New York.Google Scholar
  29. Russell, W.L., 1962, An augmenting effect of dose-fractionation on radiation-induced mutations in mice, Proc. Natl. Acad. Sci., 48: 1724.PubMedCrossRefGoogle Scholar
  30. Russell, W.L., 1963, The effect of radiation dose rate and fractionation on mutation rate in mice, in: Repair from Genetic Radiation Damage, F.J. Sobels, ed., Pergamon Press, Oxford.Google Scholar
  31. Russell, W.L., 1965c, Effect of the interval between irradiation and conception on mutation frequency in female mice, Proc. Natl. Acad. Sci., 54: 1552.PubMedCrossRefGoogle Scholar
  32. Russell, W.L., 1972, The genetic effects of radiation, in: “Peaceful Uses of Atomic Energy”, United Nations, New York.Google Scholar
  33. Russell, W.L., 1977, Mutation frequencies in mouse oocytes and the estimation of genetic hazards of radiation in women, Proc. Natl. Acad. Sci., 74: 135.CrossRefGoogle Scholar
  34. Russell, W.L., 1977, Mutation frequencies in mouse oocytes and the estimation of genetic hazards of radiation in women, Proc. Natl. Acad. Sci., 74: 3523.PubMedCrossRefGoogle Scholar
  35. Sax, K., 1940, An analysis of X-ray induced chromosomal aberrations in Tradescantia, Genetics, 25: 41.PubMedGoogle Scholar
  36. Schalet, A.P., and Sankararanarayanan, K., 1976, Evaluation and re-evaluation of genetic radiation hazards in man. I. Interspecific comparison of estimates of mutation rates. Mutat. Res., 35: 341.PubMedCrossRefGoogle Scholar
  37. Searle, A.G., 1974, Mutation induction in mice, Adv. in Radiat. Mol., 4: 151.Google Scholar
  38. Spencer, W.P., and Stern, C., 1948, Experiments to test the validity of the linear gamma dose-mutation frequency relation in Drosophila at low dosage, Genetics, 33: 43.Google Scholar
  39. Traut, H., 1963, Dose dependence of the frequency of radiation-induced recessive sex-linked lethals in Drosophila melanogaster with special consideration of the stage sensitivity of the irradiated germ cells, in: “Repair from Genetic Radiation Damage” F.H. Sobels, ed., Pergamon Press, Oxford.Google Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • S. Abrahamson
    • 1
  • H. U. Meyer
    • 1
  • C. DeJongh
    • 1
  1. 1.Department of ZoologyUniversity of WisconsinMadisonUSA

Personalised recommendations