Cell Growth pp 133-169 | Cite as

Growth Parameters in Normal and Tumor Cells: Non-Cycling Cells and Metastatic Variants as Monitored by Flow Cytometry

  • C. Nicolini
  • S. Lessin
  • S. Abraham
  • A. Chiabrera
  • S. Zietz
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 38)


Today in the biological sciences there is a growing interest and use in technologies capable of remote in vivo quantization of cellular constituents. The biophysical application of two such technologies, as scanning (1) and flow (2) cytometry, to elucidate the in vivo mechanisms of proliferation, differentiation and expression of metastatic potential in mammalian cell systems, has been recently introduced (for an updated review see also the chapters by Nicolini, Mendelsohn and Casperson in the previous book of this NATO-ASI series (3)). These technologies have made possible the detailed and quantitatively accurate characterization of “native interphase chromatin” (1,4,5) and membrane (2,6). This characterization has in turn led to a new model for normal and abnormal cell growth, that is related to and dependent upon the quinternary nuclear organization of the chromatin (1,4).


Metastatic Potential Pulmonary Metastasis Ethidium Bromide Acridine Orange Acridine Orange 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • C. Nicolini
    • 1
    • 2
  • S. Lessin
    • 1
    • 2
  • S. Abraham
    • 1
    • 2
  • A. Chiabrera
    • 1
    • 2
  • S. Zietz
    • 1
    • 2
  1. 1.Temple UniversityHealth Sciences CenterPhiladelphiaUSA
  2. 2.National Research CouncilItaly

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