Advertisement

Cell Growth pp 715-721 | Cite as

Gene Mutation, Quantitative Mutagenesis, and Mutagen Screening in Mammalian Cells: Study with the CHO/HGPRT System

  • Abraham W. Hsie
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 38)

Summary

We have employed CHO cells to develop and define a set of stringent conditions for studying mutation induction to TG resistance. Several lines of evidence support the CHO/HGPRT system as a specific-locus mutatational assay. The system permits quantification of mutation at the HGPRT locus induced by various physical and chemical mutagens. The quantitative nature of the system provides a basis for the study of structure-function relationships of various classes of chemical mutagens. The intra- and interlaboratory reproducibility of this system suggests its potential for screening environmental agents for mutagenic activity.

Keywords

Chinese Hamster Ovary Cell Chinese Hamster Ovary Mutagenic Activity Cloning Efficiency Chemical Mutagen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Puck, T. T. and Marcus, P. I. (1955) Proc. Natl. Acad. Sci. USA 41, 432–437.CrossRefGoogle Scholar
  2. 2.
    Puck, T. T. and Kao, F.-T. (1967) Proc. Natl. Acad. Sci. USA 58, 1227–1234.CrossRefGoogle Scholar
  3. 3.
    Chu, E. H. Y. and Mailing, H. V. (1968) Proc. Matl. Acad. Sci. USA 61, 1306–1312.CrossRefGoogle Scholar
  4. 4.
    Thompson, L. H. and Baker, R. M. (1973) In Prescott, D. M. (ed.), Methods in Cell Biology, Academic Press, New York, pp 209–281.Google Scholar
  5. 5.
    Siminovitch, L. (1976) Cell 7, 1–11.PubMedCrossRefGoogle Scholar
  6. 6.
    Hsie, A. W. (1980) In Williams, G. M., Kroes, R., Waaijers, H. W. and Van de Poll, K. W. (eds.), Predictive Value of Short-Term Screening Tests in Carcinogenicity Evaluation, Elsevier/North-Holland Biomedical Press, Amsterdam/New York/Oxford, pp 89–102.Google Scholar
  7. 7.
    Hsie, A. W., Brimer, P. A., Mitchell, T. J. and Gosslee, D. G. (1975) Somat. Cell Genet. 1, 247–261.PubMedCrossRefGoogle Scholar
  8. 8.
    O’Neill, J. P., Brimer, P. A., Machanoff, R., Hirsch, G. P. and Hsie, A. W. (1977) Mutat. Res. 45, 91–101.PubMedCrossRefGoogle Scholar
  9. 9.
    Machanoff, R., O’Neill, J. P., San Sebastian, J. R., Brimer, P. A. and Hsie, A. W. (1980) Proc. 11th Annu. Meet. Environ. Mutagen Soc., Mar. 16–19, 1980, Nashville, TN, p 102.Google Scholar
  10. 10.
    Hsie, A. W., O’Neill, J. P., Couch, D. B., San Sebastian, J. R., Brimer, P. A., Machanoff, R., Fuscoe, J. C., Riddle, J. C., Li, A. P., Forbes, N. L. and Hsie, A. W. (1978) Radiat. Res. 76, 471–492.PubMedCrossRefGoogle Scholar
  11. 11.
    Hsie, A. W., Brimer, P. A., Machanoff, R. and Hsie, M. H. (1977) Mutat. Res. 45, 271–282.PubMedCrossRefGoogle Scholar
  12. 12.
    Hsie, A. W., Brimer, P. A., Mitchell, T. J. and Gosslee, D. G. (1975) Somat. Cell Genet. 1, 383–389.PubMedCrossRefGoogle Scholar
  13. 13.
    O’Neill, J. P. and Hsie, A. W. (1977) Nature 269, 815–817.PubMedCrossRefGoogle Scholar
  14. 14.
    Thielman, H.-W., Schroder, C. H., O’Neill, J. P. and Hsie, A. W. (1979) Chem.-Biol. Interact. 26, 233–243.CrossRefGoogle Scholar
  15. 15.
    Johnson, N. P., Hoeschele, J. D., Rahn, R. O., O’Neill, J. P. and Hsie, A. W. (1980) Cancer Res. 40, 1463–1468.PubMedGoogle Scholar
  16. 16.
    Couch, D. B. and Hsie, A. W. (1978) Mutat. Res. 57, 209–216.PubMedCrossRefGoogle Scholar
  17. 17.
    Couch, D. B., Forbes, N. L. and Hsie, A. W. (1978) Mutat. Res. 57, 217–224.PubMedCrossRefGoogle Scholar
  18. 18.
    O’Neill, J. P., Fuscoe, J. C. and Hsie, A. W. (1978) Cancer Res. 38, 506–509.PubMedGoogle Scholar
  19. 19.
    Fuscoe, J. C., O’Neill, J. P., Peck, R. M. and Hsie, A. W. (1979) Cancer Res. 39, 4875–4881.PubMedGoogle Scholar
  20. 20.
    Tan, E.-L. and Hsie, A. W. (1980) Proc. 11th Annu. Meet. Environ. Mutagen Soc., Mar. 16–19, 1980, Nashville, TN, p 56.Google Scholar
  21. 21.
    Hsie, A. W., Brimer, P. A., O’Neill, J. P., Epier, J. L., Guerin, M. R. and Hsie, M. H. (1980) Mutat. Res. 78, 79–84.PubMedCrossRefGoogle Scholar
  22. 22.
    Hsie, A. W., O’Neill, J. P., Machanoff, R., Schenley, R. L. and Brimer, P. A. (1981) In de Serres, F. J. (ed.), International Programs for the Evaluation of Short-Term Tests for Carcinogenicity (in press).Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Abraham W. Hsie
    • 1
  1. 1.Biology DivisionOak Ridge National LaboratoryOak RidgeUSA

Personalised recommendations