Informational Macromolecules in Stationary and Dividing Hepatocytes and Hepatomas
It is a fundamental tenet of cell biology that the phenotype of the cell is a manifestation of the proteins of which it is composed and that the protein spectrum results from modulated expression of the genes coding for them. It is now clear that regulation at translational level, if it plays a part at all, plays a relatively minor part in determining the amounts of proteins made, and this leaves the implication that the phenotype of a cell is mainly determined by the population of messenger RNA molecules within it. Less than 10 years ago we had no idea of how this population varied in cells of different phenotypes or in different physiological states, and we did not know how the population was comprised. However, the development of analytical methods using nucleic acid hybridization has enabled us to establish a number of general principles and more recently the availability of techniques to prepare DNA libraries has made it possible to start to work out some details.
KeywordsNormal Liver Nucleic Acid Hybridization Globin mRNA Hepatoma Tissue Culture Cell Globin Messenger RNAs
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