Cell Cycle Phase-Specific Changes in Relaxation Times and Water Content in HeLa Cells
The ubiquity of water molecules and their essential role in life processes is well known. Although a number of quantitative methods are now available for the study of water, the structure and function of this simple molecule in solutions and in biological systems is not well understood. Much of the early work with water in biological tissues was confined primarily to simple measurements of water content of different tissues. In 1913, it was noted that the percentage of water in some tumors is higher than that of the host tissues (1). Cramer, in 1916, presented evidence supporting the notion that the percentage of water in tumors is directly related to the rate of tumor growth (2). McEwen et al. (3) later reported that the increased percentage of water in tumors was not due to the loss of dry solids. Evidence demonstrating a systemic effect of tumors on the water content of other organs was reported in 1932 by Schlottman and Rubenow (4). By 1943, this systemic effect of tumors on the percentage of water in organs was well known (1). After the 1940’s, interest in the water content of tumors seemed to wane.
KeywordsNuclear Magnetic Resonance HeLa Cell Chinese Hamster Ovary Mitotic Cell Random Population
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