Cell Growth pp 411-454 | Cite as

Chromatin Structure, Histone Modifications and the Cell Cycle

  • E. M. Bradbury
  • H. R. Matthews
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 38)

Abstract

Credit for the recent major advances in our understanding of chromatin structure must be attributed largely to the finding by Hewish and Burgoyne (1) that Ca++ activated endogenous nucleases preferentially cleaved the DNA in rat liver chromatin at sites separated by about 200 base pairs (bp)(2). This led to their proposal that chromatin was a simple repeating subunit structure. It also led to the research industry that has built up on the uses of different nucleases as biochemical probes of chromatin structure and on the additional use of staphylococcal(s.) nuclease in biochemical procedures for the preparation of large amounts of the chromatin subunit, the nucleosome, oligomers of nucleosomes and large pieces of chromatin.

Keywords

Cellulose Urea Electrophoresis Fractionation Serine 

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • E. M. Bradbury
    • 1
  • H. R. Matthews
    • 1
  1. 1.Department of Biological Chemistry School of MedicineUniversity of CaliforniaDavisUSA

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