Use of Monoclonal Antibodies to Study Cytoskeleton
The lymphocyte hybridoma technique developed by Köhler and Milstein (1975) has been widely and successfully used in the field of immunology and virology (Melchers et al., 1978; Kennett et al., 1980; Milstein and Lennox, 1980). However, there are few reports describing the monoclonal antibodies to cytoskeletal proteins. Monoclonal antibodies against cytoskeletal components have the potential not only for improving the immunofluorescent localization of specific proteins within cells but also for analyzing the functional sites of specific proteins and identifying the previously unidentified proteins. Furthermore, they can be used together with microinjection techniques (Feramisco, 1979; Lin and Feramisco, 1981) to investigate the physiological roles of specific proteins. In this chapter, we describe some examples to illustrate the advantages of monoclonal antibodies directed against cytoskeletal components.
KeywordsIntermediate Filament Human Skin Fibroblast Cytoskeletal Component Tryptic Fragment Immunofluorescent Localization
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