The Role of Tubulin in Steroidogenesis of Mouse Adrenal Y-1 Cells and Rat Leydig CCL 43 Cells

  • Mike A. Clark
  • Jerry W. Shay


The mechanisms of adrenocorticotropic hormone (ACTH)-induced steroid secretion in the adrenal cortex have not been clearly elucidated. However, it has been demonstrated that ACTH acts in the regulation of the steroidogenic pathway by controlling the conversion of cholesterol into pregnenolone. Cellular-fractionation studies have demonstrated that the enzymes necessary for this conversion are located in the mitochondria, whereas the cholesterol is stored in the cytoplasm. Kowal (1970) demonstrated that all the enzymes necessary to synthesize steroids are present prior to ACTH stimulation, and he concluded that steroidogenesis was regulated by controlling the transport of cholesterol to the mitochondria. Temple and Wolff (1973) showed that colchicine, and other drugs that depolymerize microtubules, could induce steroidogenesis independent of ACTH, and from these data they hypothesized that microtubules prevented the transport of cholesterol to the mitochondria and that the mechanism of ACTH-induced steroidogenesis involved the removal of this restriction.


Cholesterol Crystal Granular Form Organize Microtubule ACTH Treatment Steroidogenic Pathway 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Buonassi, V., Sato, G., and Cohen, A. I., 1962, Hormone producing cultures of adrenal and pituitary tumor origin, Proc. Natl. Acad. Sci. U.S.A. 48;1184.CrossRefGoogle Scholar
  2. Clark, M. A., and Shay, J. W., 1979, The response of whole and enucleated adrenal cortical tumor cells (Y-l cells) to ACTH treatment, Scanning Electron Microsc. 3;527.Google Scholar
  3. Fuller, G. M., Brinkley, B. R., and Boughten, J. M., 1975, Immunofluorescence of mitotic spindle using monospecific antibodies against bovine brain tubulin, Science 187;948.CrossRefGoogle Scholar
  4. Kowal, J., 1970, ACTH and the metabolism of adrenal cell cultures, Recent Prog. Horm. Res. 26;623.Google Scholar
  5. Lazarides, E., and Weber, K., 1974, Actin antibody and the specific visualization of actin filaments in non-muscle cells, Proc. Natl. Acad. Sci. U.S.A. 71;2268.CrossRefGoogle Scholar
  6. Mattson, P., and Kowal, J., 1978, The ultrastructure of functional mouse adrenal cortical tumor cells in vivo, Differentiation 11;75.CrossRefGoogle Scholar
  7. Schneider, E. L., Stanbridge, E. J., and Epstein, C. J., 1974, Incorporation of 3H-uridine and 3H-uracil into RNA, Exp. Cell Res. 84;311.CrossRefGoogle Scholar
  8. Tai, Y. H., and Quinn, P. A., 1977, Rapid detection of mycoplasma contamination in tissue cultures by SEM, Scanning Electron Microsc. 2;291.Google Scholar
  9. Temple, R., and Wolff, J., 1973, Stimulation of steroid secretion by antimicrotubular agents, J. Biol. Chem. 248;2691.Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Mike A. Clark
    • 1
  • Jerry W. Shay
    • 1
  1. 1.Department of Cell BiologyThe University of Texas Health Science Center at DallasDallasUSA

Personalised recommendations