Collection of Gametes in Laboratory Animals and Preparation of Sperm for In Vitro Fertilization

  • Gene Oliphant
  • Ludeman A. Eng


Ova to be used for in vitro fertilization are routinely collected after superovulation. In small animals and depending on the species, 5–150 IU of pregnant mare serum gonadotropin (PMSG) is administered 2–4 days prior to an ovulating dose (5–125 IU) of human chorionic gonadotropin (hCG) (Yanagimachi, 1969; Iwamatsu and Chang, 1969; Barros et al., 1973; Brackett and Oliphant, 1975). Superovulation procedures, for example in rabbit, can produce as many as 60 ova per rabbit but will more commonly result in 20 ± 3.0 ova recovered from the oviduct. There is some evidence that superovulation may cause chromosomal abnormalities (Fugimoto et al., 1974; Maudlin and Fraser, 1977).


Follicular Fluid Seminal Plasma High Ionic Strength Acrosome Reaction Female Reproductive Tract 


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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Gene Oliphant
    • 1
  • Ludeman A. Eng
    • 1
  1. 1.Department of AnatomyUniversity of Virginia School of MedicineCharlottesvilleUSA

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