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Blastocyst Fluid Formation

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Abstract

All mammalian blastocysts undergo some expansion prior to implantation, but the amount varies between species. Blastocysts of the human, mouse, and rat display minimal volume changes, while those of the rabbit and pig undergo enormous increases. Figure 1 presents growth curves for preimplantation rabbit blastocysts. The blastocoelic cavity increases from about 70 nl on Day 4 postcoitus (p.c.) to almost 80 μl on Day 7 p.c., the day of implantation. This increase in size stems largely from fluid accumulation, although cell multiplication also occurs (Daniel, 1964). Because the blastocyst consists almost entirely of a single-cell-thick epithelium, the trophectoderm. it is ideally suited for the study of developmental aspects of epithelial solute and water movements.

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References

  • Baker, P. F., and Willis, J. S., 1970, Potassium ions and the binding of cardiac glycosides to mammalian cells, Nature (London) 266:521–523.

    Article  Google Scholar 

  • Balaban, R. S., Mandel, L. J., Soltoff, S. P., and Storey, J. M., 1980, Coupling of active ion transport and aerobic respiratory rate in isolated renal tubules, Proc. Natl. Acad. Sci. USA 22:447–451.

    Article  Google Scholar 

  • Benos, D. J., 1981a, Developmental changes in epithelial transport characteristics of preimplantation rabbit blastocysts, J. Physiol. (London), in press.

    Google Scholar 

  • Benos, D. J., 1981b, Ouabain binding to preimplantation rabbit blastocysts, Dev. Biol. 83:69–78.

    Article  Google Scholar 

  • Benos, D. J., and Balaban, R. S., 1980. The energy requirements for active ion transport of the developing mammalian blastocyst, Biol. Reprod. 23:941–947.

    Article  Google Scholar 

  • Benos, D. J., Mandel, L. J., and Balaban, R. S., 1979, On the mechanism of the amiloridesensitive entry site interaction of anuran epithelia, J. Gen. Physiol. 73:307–326.

    Article  Google Scholar 

  • Biggers, J. D., and Powers, R. D., 1979, Na transport and swelling of the mammalian blastocyst: Effect of amiloride, in: Amiloride and Epithelial Sodium Transport (A. W. Cuthbert, G. M. Fanelli, Jr., and A. Scriabine, eds.), Urban & Schwarzenberg, Munich, pp. 167–179.

    Google Scholar 

  • Biggers, J. D., Borland, R. M., and Lechene, C. P., 1978, Ouabain-sensitive fluid accumulation and ion transport by rabbit blastocysts, J. Physiol. (London) 280:319–330.

    Google Scholar 

  • Borland, R. M., and Biggers, J. D., 1981, Water permeability of the rabbit blastocyst in vitro, manuscript in preparation.

    Google Scholar 

  • Borland, R. M., Biggers, J. D., and Lechene, C. P., 1976, Kinetic aspects of rabbit blastocoele fluid accumulation: An application of electron probe analysis, Dev. Biol. 50:201–211.

    Article  Google Scholar 

  • Borland, R. M., Biggers, J. D., and Lechene, C. P., 1977, Fluid transport by rabbit preimplantation blastocysts, J. Reprod. Fertil. 51:131–135.

    Article  Google Scholar 

  • Cross, M. H., 1971, Rabbit blastocoele perfusion technique, Nature (London) 232:635–637.

    Article  Google Scholar 

  • Cross, M. H., 1973, Active sodium and chloride transport across the rabbit blastocoele wall, Biol. Reprod. 8:566–575.

    Google Scholar 

  • Cross, M. H., 1974, Rabbit blastocoele bicarbonate: accumulation rate, Biol. Reprod. 11:654–662.

    Article  Google Scholar 

  • Cross, M. H., and Brinster, R. L., 1970, Influence of ions, inhibitors and anoxia on transtrophoblast potential of rabbit blastocysts, Exp. Cell Res. 62:303–309.

    Article  Google Scholar 

  • Daniel, J. C., 1964, Early growth of the rabbit trophoblast, Am. Nat. 98:85–98.

    Article  Google Scholar 

  • Diamond, J. M., 1977, The epithelial junction: Bridge, gate, and fence, Physiologist 20:10–18.

    Google Scholar 

  • Gamow, G., and Daniel, J. C., 1970, Fluid transport in the rabbit blastocyst, Wilhelm Roux Arch. Entwicklungsmech. Org. 164:261–278.

    Article  Google Scholar 

  • Hills, F., James, P. S., Paterson, J. Y. F., and Smith, M. W., 1980, Delayed development of amiloride-sensitive sodium transport in lamb distal colon, J. Physiol. (London) 303:371–384.

    Google Scholar 

  • House, C. R., 1974, Water Transport in Cells and Tissues, Arnold, London.

    Google Scholar 

  • Leibo, S. P., 1980, Water permeability and its activation energy of fertilized and unfertilized mouse ova, J. Membr. Biol. 53:179–188.

    Article  Google Scholar 

  • Levine, S., Franki, N., and Hays, R. M., 1973, Effect of phloretin on water and solute movement in the toad bladder, J. Clin. Invest. 52:1435–1442.

    Article  Google Scholar 

  • Mandel, L. J., and Curran, P. F., 1972, Response of the frog skin to steady-state voltage clamping. I. The shunt pathway, J. Gen. Physiol. 59:503–518.

    Article  Google Scholar 

  • Powers, R. D., Borland, R. M., and Biggers, J. D., 1977, Acquisition of amiloride-sensitive rheogenic Na+-transport in the rabbit blastocyst, Nature (London) 270:603–604.

    Article  Google Scholar 

  • Smith, M. W., 1970, Active transport in the rabbit blastocyst, Experientia 26:736–738.

    Article  Google Scholar 

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© 1981 Plenum Press, New York

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Benos, D.J., Biggers, J.D. (1981). Blastocyst Fluid Formation. In: Mastroianni, L., Biggers, J.D. (eds) Fertilization and Embryonic Development In Vitro . Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4016-4_12

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  • DOI: https://doi.org/10.1007/978-1-4684-4016-4_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4018-8

  • Online ISBN: 978-1-4684-4016-4

  • eBook Packages: Springer Book Archive

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