Blastocyst Fluid Formation

  • Dale John Benos
  • John D. Biggers


All mammalian blastocysts undergo some expansion prior to implantation, but the amount varies between species. Blastocysts of the human, mouse, and rat display minimal volume changes, while those of the rabbit and pig undergo enormous increases. Figure 1 presents growth curves for preimplantation rabbit blastocysts. The blastocoelic cavity increases from about 70 nl on Day 4 postcoitus (p.c.) to almost 80 μl on Day 7 p.c., the day of implantation. This increase in size stems largely from fluid accumulation, although cell multiplication also occurs (Daniel, 1964). Because the blastocyst consists almost entirely of a single-cell-thick epithelium, the trophectoderm. it is ideally suited for the study of developmental aspects of epithelial solute and water movements.


Fluid Accumulation Transepithelial Electrical Resistance Ouabain Binding Toad Urinary Bladder Methionine Uptake 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Dale John Benos
    • 1
  • John D. Biggers
    • 1
  1. 1.Department of Physiology and Biophysics, Laboratory of Human Reproduction and Reproductive BiologyHarvard Medical SchoolBostonUSA

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