Abstract
The emergence of R plasmids in H. influenzae is of great clinical concern, since H. influenzae causes serious infections including meningitis, epiglottitis, pneumonia, and otitis media. Resistance to ampicillin (Ap), as well as to tetracycline (Tc) was shown to be plasmid linked (1, 2). The conjugative Haemophilus R plasmids that have been described are closely related to each other and have most of their base sequences in common independent of their geographical origins and their antibiotic resistance markers (3, 4). This paper examines whether these R factors arose as a result of the transposition of different resistance genes on to closely related indigenous H. influenzae plasmids or whether closely related R factors from the same incompatibility group with different resistance genes have now infected H. influenzae strains throughout the world.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Literature
De Graaff, J., L.P. Elwell, and S. Falkow. 1976. Molecular nature of two beta-lactamase-speci-fying plasmids isolated from Haemophilus in-fluenzae type b. J. Bacteriol. 126: 439–446.
Elwell, L.P., J.R. Saunders, M.H. Richmond, and S. Falkow. 1977. Relationships between some R-plasmids found in Haemophilus influenzae. J. Bacteriol. 131: 356–362.
Laufs, R., and P.-M. Kaulfers. 1977. Molecular characterization of a plasmid specifying ampi-cillin resistance and its relationship to other R factors from Haemophilus influenzae. J. Gen. Microbiol. 103: 277–286.
Kaulfers, P.-M., R. Laufs, and G. Jahn.1978. Molecular properties of transmissible R factors of Haemophilus influenzae determining tetracycline resistance. J. Gen. Microbiol. 105: 243–252.
Jahn, G., R. Laufs, P.-M. Kaulfers, and H. Kolenda. 1979. Molecular nature of two Haemophilus in-fluenzae R factors containing resistances and the multiple integration of drug resistance transposons. J. Bacteriol. 138: 584–597.
Heffron, F., C. Rubens, and S. Falkow. 1977. Transposition of a plasmid deoxyribonucleic acid sequence that mediates ampicillin resistance: identity of laboratory-constructed plasmids and clinical isolates. J. Bacteriol. 129: 530–533.
Crosa, J.H., J. Brenner, and S. Falkow. 1973. Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo-and heteroduplexes. J. Bacteriol. 115: 904–911.
Timmis, K.N., F. Cabello, and S.N. Cohen. 1978. Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol. Gen. Genet. 162: 121–137.
Kleckner, N., J.A. Swan, and M. Zabeau. 1978. Properties of the translocatable tetracycline resistance element Tn10 in Escherichia coli and bacteriophage lambda. Genetics 90: 427–461.
Alton, N.K., and D. Vapnek. 1979. Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9. Nature 282: 1–6.
Rosner, J.L., and M.M. Gottesman. 1977. Transposition and deletion of Tn9: a transferable element carrying the gene for chloramphenicol resistance, p. 213–218. In: A.I. Bukhari, J.A. Shapiro, and S.L. Adhya (ed.), DNA insertion elements, plasmids, and episomes. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Stuy, J.H. 1980. Chromosomally integrated conjugative plasmids are common in antibiotic-resistant Haemophilus influenzae. J. Bacteriol. 142: 925–930.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1981 Springer Science+Business Media New York
About this chapter
Cite this chapter
Laufs, R., Fock, R., Kaulfers, PM. (1981). Studies on Drug Resistance Transposons in Haemophilus influenzae R Plasmids. In: Levy, S.B., Clowes, R.C., Koenig, E.L. (eds) Molecular Biology, Pathogenicity, and Ecology of Bacterial Plasmids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3983-0_8
Download citation
DOI: https://doi.org/10.1007/978-1-4684-3983-0_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-3985-4
Online ISBN: 978-1-4684-3983-0
eBook Packages: Springer Book Archive