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Metabolic Plasmid Organization and Distribution

  • I. C. Gunsalus
  • K.-M. Yen

Summary

Pseudomonas strains carry plasmids under regulation of natural and synthetic organic residues and bear primary roles in mineralization. Aromatic compounds of known oxidation pathways provide convenient models for genetic analyses and for plasmid DNA isolation and structure determination. The alkane and terpene catabolic systems, coded on larger self-fertile plasmids, have provided primary data on gene organization and regulation, as well as plasmid chromosome gene redundancy.

Two aromatic plasmids, HAH7 and SAL1, of about 80 to 90 kb (kilobases), code respectively the conversion of naphthalene and of salicylate to the anaplerotic intermediates, pyruvate and acetaldehyde, plus CO, or formate, thus supporting cell growth. The NAH plasmid codes for these two conversions on separate operons, both controlled by salicylate or anthrinilate. Operon 1 codes the conversion of naphthalene to salicylate; operon 2, salicylate via catechol with “meta“ (2,3 oxygenase) aromatic ring cleavage. Plasmid DNA isolated from wild type and transposon Tn5 induced insertion mutants was scored for defective loci by enzyme assays in the genomes subjected to gel electrophoresis after restriction digestion. An EcoRl digest fragment A of 23 kilobases carries the bulk of both operons; Smal yields 5 fragments, A of 42 kilobases and B of 18. The latter which lacks the left hand 5+ kilobases of EcoRl A reveals that the replicon in the nahA gene are within this 5 kilobase region. The transcription is from left to right in both operons; an 8 to 10 kilobase segment between the operons carries at least one regulatory locus. The cell plasmid in Smal digest yields 5 fragments identical in size to those of NAH7, plus two smaller, about 3 kb, segments which constitute an insertion in naphthalene operon 1 in the gene AB region. The methuds now available for plasmid isolation and DNA analyses, the genetic scoring and cloning, now appear capable of providing, in the near future, a complete structure, organization, and regulation model of the aromatic plasmids in fluorescent Pseudontonas species.

Keywords

Fluorescent Pseudomonad Ring Fission Catechol Dioxygenase Naphthalene Oxidation Catabolic Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    P. H. Clarke and M. H. Richmond, Evolutionary Prospects for Pseudomonas species, in: “Genetics and Biochemistry of Pseudomonas,” P. H. Clarke and M. H. Richmond, eds., John Wiley & Sons, New York, 1975.Google Scholar
  2. 2.
    A. M. Chakrabarty, Plasmids in Pseudomonas, in: “Annual Reviews of Genetics,” H. L. Roman, A. Campbell, and L. M. Sandler, eds., Vol. 10, Annual Reviews, Inc., Palo Alto, Ca., 1976.Google Scholar
  3. 3.
    P. A. Williams, Catabolic plasmids, TIBS, 6: 23, 1981.Google Scholar
  4. 4.
    P. H. Clarke and M. H. Richmond, eds., “Resistance of Pseudomonas aeruginosa,” John Wiley & Sons, New York, 1975.Google Scholar
  5. 5.
    G. A. Jacoby, Classification of Plasmids in Pseudomonas aeruginosa, Microbiology-1977, D. Schlessinger, ed., American Society for Microbiology, 1977.Google Scholar
  6. 6.
    J. B. Johnston and I. C. Gunsalus, Isolation of Metabolic Plasmids DNA from Pseudomonas putida, Biochem. Biophys. Res. Commun., 75: 13, 1977.PubMedCrossRefGoogle Scholar
  7. 7.
    R. Farrell, Ph.D. Thesis, Biochemistry Department, University of Illinois, Urbana, 1979.Google Scholar
  8. 8.
    R. Farrell, and A. M. Chakrabarty, Degradative Plasmids: Molecular Nature and Mode of Evolution, in: “Plasmids of Medical, Environmental and Commercial Importance,” K. N. Timmis and A. Punier, eds., Elsevier/North Holland Biomedical Press, 1979.Google Scholar
  9. 9.
    N. J. Palleroni, General Properties and Taxonomy of the Genus Pseudomonas, in: “Genetics and Biochemistry of Pseudomonas,” P. H. Clarke and M. H. Richmond, eds., John Wiley & Sons, New York, 1975.Google Scholar
  10. 10.
    R. A. Jorgensen, S. J. Rothstein, and W. S. Reznikoff, A Restriction Enzyme Cleavage Map of Tn5 and Location of a Region Encoding Neomycin Resistance, Molec. Gen. Genet., 177: 65, 1979.Google Scholar
  11. 11.
    W. K. Yeh and D. T. Gibson, Resolution of toluene dioxygenase into three separate protein components, Bact. Proc, p. 166, 1974.Google Scholar
  12. 12.
    S. Dagley, Pathways for the Utilization of Organic Growth Substrates, in: “The Bacteria,” Vol. VI, I. C. Gunsalus, ed., Academic Press, New York, 1978.Google Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • I. C. Gunsalus
    • 1
  • K.-M. Yen
    • 1
  1. 1.Biochemistry DepartmentUniversity of IllinoisUrbanaUSA

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