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Development of Broad Host-Range Plasmid Vectors

  • Peter T. Barth
  • Lyn Tobin
  • Geoffrey S. Sharpe

Abstract

The majority of cloning vectors developed so far are based on Plasmids or phages with a narrow host-range such as ColEI(pBR3221), P15A(pACYC1842),pSC1013 or phage λ4. These are limited to Escherichia coli or closely related enterobacterial species. For the genetic analysis and manipulation of a wider range of micro-organisms, including those of agricultural, medical, environmental or industrial importance, use has been made of plasmids belonging to the IncP group such as RP4 or RK2. For example RP4 has unique cloning sites for EcoRI, BamHI, HindIII, HpaI and BglII5,6 and has been converted into a SalI cloning vector pRP3017,8. From RK2 a two vehicle system: pRK290 plus pRK2013, has been constructed9. But such vectors remain relatively large, have a low copy number and are therefore unsuitable for some cloning purposes. For ease of manipulation and high gene dosage of the cloned material we need small, high copy number, broad host-range plasmids.

Keywords

High Copy Number Acinetobacter Calcoaceticus PstI Fragment Insertional Inactivation HpaI Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Peter T. Barth
    • 1
  • Lyn Tobin
    • 1
  • Geoffrey S. Sharpe
    • 2
  1. 1.ICI Corporate LaboratoryThe HeathCheshireEngland
  2. 2.ICI Joint LaboratoryUniversity of LeicesterLeicesterEngland

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