Isolation and Analysis of a Cosmid Hybrid Containing the Human Genomic Interferon Gene, HuIFNβ1
Human fibroblast interferon HuIFNβ has an anti-viral activity and can also stimulate natural killer cell action against neoplastic cells1 2 3. The IFNβ-gene belongs to a rare class of eukaryotic genes for which the immediate induction of transcription in response to certain inducers such as poly I:poly C has been demonstrated4 5 6. Recent findings indicate that two IFNβ mRNAs exist which are at the most only distantly related, but are co-ordinately induced in human fibroblasts7. It therefore seemed of great interest to isolate the chromosomal region for the IFNß1 gene so as to study the structure of the transcription unit, the possible adjacent transcription units, and the later application of this information to the production of interferon ß in eukaryotic cells.
KeywordsNatural Killer Cell Action EcoRI Fragment Cosmid Cloning PstI Site Terminal Transferase
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