Plasmid Mobilization as a Tool for in Vivo Genetic Engineering

  • J. Leemans
  • D. Inzé
  • R. Villarroel
  • G. Engler
  • J. P. Hernalsteens
  • M. De Block
  • M. Van Montagu


Mutagenesis through the insertion of transposons has proved to be an invaluable technique for mapping the genes of complex plasmids1. No selection for a mutant phenotype has to be devised, but a straightforward selection for the antibiotic resistance markers, encoded by the transposon, is sufficient to identify the presence of a mutant plasmid.


Transposable Element Plasmid Transfer Conjugative Plasmid Mutant Plasmid Heteroduplex Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    M. Holsters, B. Silva, F. Van Vliet, C. Genetello, M.De Block, P. Dhaese, A. Depicker, D. Inzé, G. Engler, R. Villarroel, M. Van Montagu and J. Schell, The functional organization of the nopaline A. tumefaciens plasmid pTiC58, Plasmid 3: 212 (1980).PubMedCrossRefGoogle Scholar
  2. 2.
    N. Datta, R.W. Hedges, E.J. Shaw, R.B. Sykes and M.H. Richmond, Properties of an R-factor from Pseudomonas aeruginosa, J. Bacteriol., 108: 1244 (1971).PubMedGoogle Scholar
  3. 3.
    J.P. Hernalsteens, R. Villarroel-Mandiola, M. Van Montagu and J. Schell, Transposition of Tnl to a broad host range drug resistance plasmid, in: “DNA Insertion Elements, Plasmids, and Episomes”, A.I. Bukhari, J.A. Shapiro, and S.L. Adhya, eds., Cold Spring Harbor Laboratory, New York (1977).Google Scholar
  4. 4.
    A.J. Clark and G.J. Warren, Conjugation transmission of Plasmids, Ann. Rev. Genet. 13: 99 (1979).PubMedCrossRefGoogle Scholar
  5. 5.
    G.J. Warren, A.J. Twigg and D.J. Sherratt, ColEl plasmid mobility and relaxation complex, Nature 274: 259 (1978).PubMedCrossRefGoogle Scholar
  6. 6.
    M. De Wilde, A. Depicker, G. De Vos, M. De Beuckeleer, E. Van Haute, M. Van Montagu and J. Schell, Molecular cloning as tool to the analysis of the Ti-plasmids of Agrobacterium tumefaciens, Ann. Microbiol. (Inst. Pasteur) 129B: 531 (1978).Google Scholar
  7. 7.
    Unpublished results of the authors.Google Scholar
  8. 8.
    T. Watanabe, H. Nishida, C. Ogata, T. Arai and S. Sato, Episome mediated transfer of drug resistance in Entero-bacteriaceae: VII. Two types of naturally occurring R factors, J. Bacteriol. 88: 716 (1964).PubMedGoogle Scholar
  9. 9.
    N.D.F. Grindley, J.N. Grindley and E.S. Anderson, R factor compatibility groups, Molec. Gen. Genet. 119: 287 (1972).PubMedGoogle Scholar
  10. 10.
    J.P. Hernalsteens, M. Holsters, A. Silva, F.Van Vliet, R. Villarroel, G. Engler, M. Van Montagu and J. Schell, A technique for mutagenesis by transposon insertion, applicable to most Gram-negative bacteria, Arch. Intern. Physiol. Biochim. 86: 432 (1978).Google Scholar
  11. 11.
    A. Depicker, M.De Block, D. Inzé, M. Van Montagu and J. Schell, IS-like element IS8 in RP4 plasmid and its involvement in cointegration, Gene 10: 329 (1980).PubMedCrossRefGoogle Scholar
  12. 12.
    J. Leemans, R. Villarroel, R. Maenhaut, G. Engler, R.W. Hedges and M. Van Montagu, Heteroduplex analysis of P-type plasmids: the role of insertion and deletion of transposable elements, (submitted).Google Scholar
  13. 13.
    A. Depicker, M.De Wilde, G.De Vos, R.De Vos, M. Van Montagu and J. Schell, Molecular cloning of overlapping segments of the nopaline Ti-plasmids pTiC58 as a means to restriction endonuclease mapping, Plasmid 3: 193 (1980).PubMedCrossRefGoogle Scholar
  14. 14.
    M. Van Montagu, J. Schell, M. Holsters, H.De Greve, J. Leemans, J.P. Hernalsteens, L. Willmitzer and L. Otten, Transfer, maintenance and expression of genes introduced into plant cells via the Ti plasmid, in:“Molecular Biology, Pathogenicity and Ecology of Bacterial Plasmids,” S.B. Levy, ed., Plenum Press, New York (1981).Google Scholar
  15. 15.
    P.J.J. Hooykaas, H. Den Dulck-Ras and R.A. Schilperoort, Molecular mechanism of Ti-plasmid mobilization by R-plasmids. Isolation of Ti-plasmids with transposons insertion in Agrobacterium tumefaciens, Plasmid 4: 64 (1980).PubMedCrossRefGoogle Scholar
  16. 16.
    Dr. R.W. Hedges, personal communication.Google Scholar
  17. 17.
    A. Depicker, M. Van Montagu and J. Schell, Physical map of RP4, in: “DNA Insertion Elements, Plasmids, and Episome s”, A.I. Bukhari, J.A. Shapiro, and S.L. Adhya, eds., Cold Spring Harbor Laboratory, New York (1977).Google Scholar
  18. 18.
    R.W. Hedges, A. Jacob and J.T. Smith, Properties of an R factor from Bordetella bronchiseptica, J. Gen. Microbiol. 84: 199 (1974).PubMedCrossRefGoogle Scholar
  19. 19.
    R.W. Hedges, V. Rodriguez-Lemoine and N. Datta, R factors from Serratia marcescens, J. Gen. Microbiol. 86: 88 (1975).PubMedCrossRefGoogle Scholar
  20. 20.
    R.W. Hedges and M. Matthew, Acquisition by Escherichia coli of plasmid-bone β lactamases normally confined to Pseudomonas spp., Plasmid 2: 269 (1979).PubMedCrossRefGoogle Scholar
  21. 21.
    D.I. Smith, R. Gomez Lus, M. Rubio Calvo, N. Datta, A.E. Jacob and R.W. Hedges, Third type of plasmid conferring gentamicin resistance in Pseudomonas aeruginosa, Antimicrob. Agents Chemother. 8: 227 (1975).PubMedCrossRefGoogle Scholar
  22. 22.
    V.A. Stanisich and J.M. Ortiz, Similarities between plasmids of the P incompatibility group derived from different bacterial genera, J. Gen. Microbiol. 94: 281 (1976).PubMedCrossRefGoogle Scholar
  23. 23.
    R.W. Hedges, M. Matthew, D.I. Smith, J.M. Cresswell and A.E. Jacob, Properties of a transposon conferring resistance to pennicillins and streptomycin, Gene 1: 241 (1977).PubMedCrossRefGoogle Scholar
  24. 24.
    C.E. Rubens, W.F. McNeill and W.E. Farrar, A transposable plasmid DNA sequence in Pseudomonas aeruginosa which mediates resistance to gentamicin and four other antimicrobial agents, J. Bacteriol. 139: 877 (1979).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • J. Leemans
    • 2
  • D. Inzé
    • 1
  • R. Villarroel
    • 1
  • G. Engler
    • 1
  • J. P. Hernalsteens
    • 2
  • M. De Block
    • 1
  • M. Van Montagu
    • 1
    • 2
  1. 1.Laboratory of GeneticsRijksuniversiteit GentGentBelgium
  2. 2.Laboratory GEVIVrije Universiteit BrusselGentBelgium

Personalised recommendations