Advertisement

Transposition and Rearrangements in Plasmid Evolution

  • C. J. Muster
  • L. A. MacHattie
  • J. A. Shapiro

Summary

Transposable elements participate in two classes of replicative recombination events: (i) full transposition; and (ii) genome rearrangements. Transpositions mobilize DNA sequences internal to the transposable element, while rearrangements also mobilize sequences external to the transposable element. We will illustrate several ways in which rearrangements mediated by IS1 and mutant Tnl elements lead to the formation of new plasmid replicons. The effect of the Tnl/Tn3 tnpR + gene product leads to dissolution of new replicons formed by Tnl-mediated rearrangements. Replicons formed by IS1-mediated rearrangements are much more stable. This result indicates that IS1 employs a different pathway to full transposition. Thus, there are at least two classes of transposable elements in bacteria which play different roles in plasmid evolution.

Keywords

Transposable Element Cold Spring Harbor Genome Rearrangement Chloramphenicol Acetyl Transferase Plasmid Replicon 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    N. D. F. Grindley and D. Sherratt, Cold Spring Harbor Symp. Quant. Biol. 43;1257 (1979).PubMedCrossRefGoogle Scholar
  2. 2.
    J. A. Shapiro, Proc. Natl. Acad. Sci. USA 76;1933 (1979).PubMedCrossRefGoogle Scholar
  3. 3.
    M. P. Calos and J. H. Miller, Cell 20: 579 (1980).PubMedCrossRefGoogle Scholar
  4. 4.
    C. J. Muster and J. A. Shapiro, Cold Spring Harbor Symp. Quant. Biol. 45:(in press).Google Scholar
  5. 5.
    R. Gill, F. Heffron, G. Dougan, and S. Falkow, J. Bacteriol. 136: 742 (1978).PubMedGoogle Scholar
  6. 6.
    F. Heffron, B. J. McCarthy, N. Ohtsubo, and E. Ohtsubo, Cell 18: 1153 (1979).PubMedCrossRefGoogle Scholar
  7. 7.
    A. Arthur and D. Sherratt, Mol. Gen. Genet. 175: 267 (1979).PubMedCrossRefGoogle Scholar
  8. 8.
    L. A. MacHattie and J. B. Jackowski, in:“DNA Insertion Elements, Plasmids, and Episomes,” A. I. Bukhari, J. A. Shapiro, and S. L. Adhya, eds., Cold Spring Harbor Laboratory, Cold Spring Harbor (1977).Google Scholar
  9. 9.
    P. J. Kretschmer and S. N. Cohen, J. Bacteriol. 139: 515 (1979).PubMedGoogle Scholar
  10. 10.
    S. N. Cohen and J. A. Shapiro, Sci. Am. 242: 40 (1980).PubMedCrossRefGoogle Scholar
  11. 11.
    R. Gill, F. Heffron, and S. Falkow, Nature 282: 797 (1979).PubMedCrossRefGoogle Scholar
  12. 12.
    L. A. MacHattie and J. A. Shapiro, Proc. Natl. Acad. Sci. USA 75: 1490 (1978).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • C. J. Muster
    • 1
  • L. A. MacHattie
    • 1
  • J. A. Shapiro
    • 1
  1. 1.Department of MicrobiologyUniversity of ChicagoChicagoUSA

Personalised recommendations