Plasmid Mini-F Encoded Functions Involved in Replication and Incompatibility
The F factor of Escherichia coli is one of the most extensively studied plasmids. It belongs to the class of plasmids with a stringent mode of replication i.e. F is normally present in a cell in only 1-2 copies per chromosome1. In order to maintain the low copy number the replication must be tightly regulated, a notion which predicts that two different F’ should not coexist in a bacterium. This has been experimentally proven and the phenomenon was termed incompatibility2,3.Thus incompatibility of two isogenic or related plasmids of the same incompatibility group may result from specific mechanisms engaged in the regulation of replication and partitioning during cell division.
KeywordsHelper Plasmid BamHI Fragment Boxed Region Cumulative Histogram PstI Fragment
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