Replication and Incompatibility Functions in Mini-F Plasmids

  • Bruce Kline
  • Ralph Seelke
  • John Trawick


One common approach to building a model for plasmid maintenance is to identify, map and characterize the genes involved in this process. The two major features of maintenance are plasmid replication and partitioning to daughter cells. As depicted elsewhere in this text, the essential components of replication appear to be at least a fixed origin of replication (ori), one to two plasmid-specified gene products for replication (rep) and a copy number control gene (cop) that appears to exert negative control. So too, plasmid F, the classic conjugal plasmid of Escherichia coli, appears to fit this general model of replication. However, F may have a more complex genetic organization for this process than other plasmids.


Acridine Orange Copy Number Increase BamHI Fragment Normal Maintenance Plasmid Maintenance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Bruce Kline
    • 1
  • Ralph Seelke
    • 1
  • John Trawick
    • 1
  1. 1.Department of Cell Biology/Section of MicrobiologyMayo Clinic RochesterUSA

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