Replication and Incompatibility Functions in Mini-F Plasmids
One common approach to building a model for plasmid maintenance is to identify, map and characterize the genes involved in this process. The two major features of maintenance are plasmid replication and partitioning to daughter cells. As depicted elsewhere in this text, the essential components of replication appear to be at least a fixed origin of replication (ori), one to two plasmid-specified gene products for replication (rep) and a copy number control gene (cop) that appears to exert negative control. So too, plasmid F, the classic conjugal plasmid of Escherichia coli, appears to fit this general model of replication. However, F may have a more complex genetic organization for this process than other plasmids.
KeywordsAcridine Orange Copy Number Increase BamHI Fragment Normal Maintenance Plasmid Maintenance
Unable to display preview. Download preview PDF.
- Figurski, D., Kolter, R., Meyer, R., Kahn, M., Eichenlaub, R., and Helinski, D. R., 1978, Replication regions of plasmids ColEl, F, R6K and RK2, in:“Microbiology-1978,” D. Schlessinger, ed., American Society for Microbiology, Washington, D.C.Google Scholar
- Kline, B., and Palchaudhuri, S., 1980, Genetic studies of F plasmid maintenance genes, Plasmid, 4:in press.Google Scholar
- Wechsler, J., and Kline, B. C., Mutation and identification of the F plasmid locus determining resistance to acridine orange curing, Plasmid, 4:in press.Google Scholar