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Genetic Organization and Expression of Non-Conjugative Plasmids

  • H. John
  • J. Nijkamp
  • Eduard Veltkamp

Abstract

Non-conjugative plasmids are plasmids that are not able to transfer themselves to other cells without the help of a conjugative system provided by the large, so-called conjugative plasmids. Non-conjugative plasmids are small plasmids. Their M.W. generally does not exceed 10 Megadaltons. Furthermore, they are multicopy plasmids; that means that they are usually present to the extend of 10-20 copies per chromosome. As all other small DNA molecules, the non-conjugative plasmids are very attractive for basic research. Over the past ten years studies on plasmids were focused on basic questions dealing with gene-function, gene-organisation, gene-expression, mechanism and control of replication, and plasmid mobilisation. And, ever since it became apparent that plasmids are a very useful tool in genetic engineering also a lot of work has been done on the construction of appropriate vector molecules.

Keywords

Plasmid Copy Number Genetic Organization Conjugative Plasmid Immunity Protein Plasmid Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • H. John
    • 1
  • J. Nijkamp
    • 1
  • Eduard Veltkamp
    • 1
  1. 1.Department of GeneticsBiological Laboratory De BoelelaanAmsterdamThe Netherlands

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