Conjugation and Resistance Transfer in Streptococci and Other Gram Positive Species: Plasmids, Sex Pheromones and “Conjugative Transposons” (A Review)

  • Don B. Clewell


Until recently, information on the nature of conjugation and related gene transfer in Gram positive bacteria has been relatively scarce. Although conjugation among the actinomycetes has been known for many years,1,2 fertility plasmids have, so far, been recognized in only a single strain of a single species in this group. Strepto-myces coelicolor strain A3(2) harbors two conjugative plasmids, SCP1 and SCP23-5; and whereas SCP2 has been isolated and characterized5,6, efforts to isolate SCP1 have been unsuccessful. The latter determines the synthesis of the antibiotic methylenomyein7 and, when integrated into the bacterial chromosome, will mobilize chromosomal segments to SCP1 strains with almost 100 percent efficiency8.


Binding Substance Clostridium Perfringens Plasmid Transfer Conjugal Transfer Aggregation Substance 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    D. A. Hopwood, Ann. N.Y. Acad. Sci. 81: 887 (1959).PubMedCrossRefGoogle Scholar
  2. 2.
    D. A. Hopwood and M. J. Merrick, Bacteriol. Rev. 41: 595 (1977).PubMedGoogle Scholar
  3. 3.
    A. Vivian, J. Gen. Microbiol. 69: 353 (1971).CrossRefGoogle Scholar
  4. 4.
    D, A. Hopwood, K. F. Chater, J. E. Dowding, and A. Vivian, Bacteriol. Rev. 37: 371 (1973).PubMedGoogle Scholar
  5. 5.
    M. J. Bibb, R. F. Freeman, and D. A. Hopwood, Mol. Gen. Genet. 154: 155 (1977).CrossRefGoogle Scholar
  6. 6.
    H. Schrempf, H. Bujard, D. A. Hopwood, and W. Goebel, J. Bacteriol. 121: 416 (1975).PubMedGoogle Scholar
  7. 7.
    L. F. Wright, and D. A. Hopwood, J. Gen. Microbiol. 95: 96 (1976).PubMedCrossRefGoogle Scholar
  8. 8.
    D. A, Hopwood, and H. M. Wright, in: “Second International Symposium on the Genetics of Industrial Microorganisms”, K. D. MacDonald, ed., Academic Press, London, New York, and San Francisco (1976).Google Scholar
  9. 9.
    A. Jacob and S. J. Hobbs, J. Bacteriol. 117: 360 (1974).PubMedGoogle Scholar
  10. 10.
    R. E. Raycroft and L. N. Zimmerman, J. Bacteriol. 87: 799 (1964).PubMedGoogle Scholar
  11. 11.
    T. Tomura, T. Hirano, T. Ito, and M. Yoshioka, Jpn. J. Microbiol, 17: 445 (1973).PubMedGoogle Scholar
  12. 12.
    G. Dunny and D. Clewell, J. Bacteriol. 124: 784 (1975).PubMedGoogle Scholar
  13. 13.
    A. Jacob, G. I. Douglas and S. J. Hobbs, J, Bacteriol. 121: 863 (1975).Google Scholar
  14. 14.
    M. L. Frazier, and L. N. Zimmerman, J. Bacteriol. 130: 1064 (1977).PubMedGoogle Scholar
  15. 15.
    D. Oliver, B. Brown and D. Clewell, J. Bacteriol. 130: 948 (1977).PubMedGoogle Scholar
  16. 16.
    P. Tomich, F. An, S. Damle and D. Clewell, Antimicrob. Ag. Chemother. 15: 828 (1979).CrossRefGoogle Scholar
  17. 17.
    T. D. Brock, and J. M. Davie, J. Bacteriol. 86: 708 (1963).PubMedGoogle Scholar
  18. 18.
    P. A. Granato and R. W. Jackson, J. Bacteriol. 100: 865 (1969).PubMedGoogle Scholar
  19. 19.
    G. Dunny, B. Brown and D. Clewell, Proc. Nat. Acad. Sci. USA 75: 3479 (1978).PubMedCrossRefGoogle Scholar
  20. 20.
    J. van Embden, H. Engel, and B. van Klingeren, Antimicrob. Ag. Chemother. 11: 925 (1977).CrossRefGoogle Scholar
  21. 21.
    H. Marder and F. H. Kayser, Antimicrob. Ag. Chemother. 12: 261 (1977).CrossRefGoogle Scholar
  22. 22.
    P. Courvalin, C. Carlier and E. Collatz, J. Bacteriol. 143: 541 (1980).PubMedGoogle Scholar
  23. 23.
    D. Krogstad, T. R. Korfhagen, R. C. Moellering, Jr., C. Wennersten, and M. N. Swartz, J. Clin. Invest. 61: 1645 (1978).PubMedCrossRefGoogle Scholar
  24. 24.
    T. Horodniceanu, L. Bougueleret, N. El-Solh, G. Bieth, and F. Delbos, Antimicrob. Ag. Chemother. 16: 686 (1979).CrossRefGoogle Scholar
  25. 25.
    J. D. A. van Embden, N. Soedirman, and H. Engel, Lancet i: 655 (1978).CrossRefGoogle Scholar
  26. 26.
    H. Engel, N. Soedirman, J. Rost, W. van Leeuwen and J. D. A. van Embden, J. Bacteriol. 142: 407–413 (1980).PubMedGoogle Scholar
  27. 27.
    E. Romero, M. Perduca, and L. Pagani, Microbiologica 2: 421 (1979).Google Scholar
  28. 28.
    H. Malke, FEMS Microbiol. Lett. 5: 335 (1979).CrossRefGoogle Scholar
  29. 29.
    D. Behnke, V. I. Golubkov, H. Malke, A. Boitsov, and A. Totolian, FEMS Microbiol. Lett. 6: 5 (1979).CrossRefGoogle Scholar
  30. 30.
    D. Clewell and A. Franke, Antimicrob. Ag. Chemother. 5: 534 (1974).CrossRefGoogle Scholar
  31. 31.
    V. Hershfield, Plasmid 2: 137 (1979).PubMedCrossRefGoogle Scholar
  32. 32.
    T. Horodniceanu, L. Boogueleret, N. El-Solh, D. Bouanchaud and Y. Chabbert, Plasmid 2: 197 (1979).PubMedCrossRefGoogle Scholar
  33. 33.
    T. Horodniceanu, D. Bouanchaud, G. Biet and Y. Chabbert, Antimicrob. Ag. Chemother 10: 795 (1976).CrossRefGoogle Scholar
  34. 34.
    N. El-Solh, D. H. Bouanchaud, T. Horodniceanu, A. F. Roussel, and Y. A. Chabbert, Antimicrob. Ag. Chemother. 14: 19 (1978).CrossRefGoogle Scholar
  35. 35.
    M. J. Gasson and F. L. Davies, J. Bacteriol. 143: 1260 (1980).PubMedGoogle Scholar
  36. 36.
    L. L. McKay, K. A. Baldwin, and P. M. Walsh, Appl. Env. Microbiol. 40: 84 (1980).Google Scholar
  37. 37.
    L. Bougueleret, G. Bieth, and T. Horodniceanu, J. Bacteriol., in press (1981).Google Scholar
  38. 38.
    G. Brefort, M. Magot, H. Ionesco, and M. Sebald, Plasmid 1: 52 (1977).PubMedCrossRefGoogle Scholar
  39. 39.
    J. I. Rood, V. N. Scott, and C. L. Duncan, Plasmid 1: 563 (1978).PubMedCrossRefGoogle Scholar
  40. 40.
    D. Oliver, B. Brown, and D. Clewell, J. Bacteriol. 130: 759 (1977).PubMedGoogle Scholar
  41. 41.
    A. Franke, G. Dunny, B. Brown, F. An, D. Oliver, S. Damle, and D. Clewell, in: “Microbiology 1978”, D. Schlessinger, ed., Am. Soc. Microbiol., Washington, D.C. (1978).Google Scholar
  42. 42.
    A. Franke and D. B. Clewell, J. Bacteriol. 145: 494 (1981).PubMedGoogle Scholar
  43. 43.
    D. Clewell, Y. Yagi, G. Dunny and S. Schultz, J. Bacteriol. 117: 283 (1974).PubMedGoogle Scholar
  44. 44.
    D. J. LeBlanc, R. J. Hawley, L. N. Lee, and E. J. St. Martin, Proc. Nat. Acad. Sci. USA 75: 3484 (1978).CrossRefGoogle Scholar
  45. 45.
    M. J. Gasson and F. L. Davies, FEMS Lett. 7: 51 (1980).CrossRefGoogle Scholar
  46. 46.
    E. M. Gibson, N. M. Chace, S. B. London, and J. London, J. Bacteriol. 137: 614 (1979).PubMedGoogle Scholar
  47. 47.
    D.Schaberg, D. Clewell, L. Glatzer, This volume.Google Scholar
  48. 48.
    F. L. Macrina, K. R. Jones, and P. H. Wood, J. Bacteriol. 143: 1425 (1980).PubMedGoogle Scholar
  49. 49.
    P. M. Courvalin, C. Carlier, O. Croissant, and D. Blangy, Molec. Gen. Genet. 132: 181 (1974).PubMedCrossRefGoogle Scholar
  50. 49a.
    M. M. Corb and M. L. Murray, FEMS Microbiol. Lett. 1: 351 (1977).CrossRefGoogle Scholar
  51. 50.
    H. Malke, H. E. Jacob, and K. Storl, Molec. & Gen. Genetics 144: 333 (1976).CrossRefGoogle Scholar
  52. 51.
    Y. Yagi, A. Franke and D. Clewell, Antimicrob. Ag. Chemother. 7: 871 (1975).CrossRefGoogle Scholar
  53. 52.
    B. Weisblum, S. Holder and S. Hailing, J. Bacteriol. 138: 990 (1979).PubMedGoogle Scholar
  54. 52a.
    M. D. Smith, N. B. Shoemaker, V. Burdett, and W. R. Guild, Plasmid 3: 70 (1980).PubMedCrossRefGoogle Scholar
  55. 53.
    G. Dunny, R. Craig, R. Carron and D. Clewell, Plasmid 2: 454 (1979).PubMedCrossRefGoogle Scholar
  56. 54.
    D. Clewell, R. Craig, G. Dunny, R. Carron and B. Brown, in: “Plasmids and Transposons: Environmental Effects and Maintenance Mechanisms”, C. Stuttard and K. R. Rozee, eds., Academic Press, Inc., N. Y. (1980).Google Scholar
  57. 55.
    D. Clewell and B. Brown, J. Bacteriol. 143: 1063 (1980).PubMedGoogle Scholar
  58. 56.
    P. Tomich and D. Clewell, Cold Spr. Harb. Symp. Quant. Biol. 43: 1217 (1978).CrossRefGoogle Scholar
  59. 57.
    P. Tomich, F. An and D. Clewell, J. Bacteriol. 141: 1366 (1980).PubMedGoogle Scholar
  60. 58.
    M. Achtman, and R. Skurray, in: “Microbial Interactions (Series B, Receptors and Recognition, Vol. 3)”, J. L. Reissig, ed., Chapman and Hall, London (1977).Google Scholar
  61. 59.
    N. Willets and R. Skurray, Ann. Rev. Genet. 14: 41 (1980).CrossRefGoogle Scholar
  62. 60.
    Y. Yagi, R. Kessler, B. Brown, D. Lopatin and D. Clewell, This volume.Google Scholar
  63. 61.
    D. J. Krogstad, R. M. Smith, R. C. Moellering, Jr., and A. R. Parquette, J. Bacteriol. 141: 963 (1980).PubMedGoogle Scholar
  64. 62.
    G. Dunny, Ph.D. Thesis, Univ. of Michigan (1978).Google Scholar
  65. 63.
    Center for Disease Control, Morbidity and Mortality Weekly Rep. 26: 285 (1977).Google Scholar
  66. 64.
    M. Jacobs, H. Koornhof, R. Robins-Browne, C. Stevenson, I. Freiman, M. Miller, M. Witcomb, M. Isaacson, J. Ward and R. Austrian, N. Eng. J. Med. 299: 735 (1978).CrossRefGoogle Scholar
  67. 65.
    A. Dang-Van, G. Tiraby, J. Acar, W. Shaw, and D. Bouanchaud, Antimicrob. Ag. Chemother. 13: 577 (1978).CrossRefGoogle Scholar
  68. 66.
    R. M. Robins-Browne, M. Gaspar, J. Ward, I. Wachsmuth, H. Koornhof, M. Jacobs and C. Thornsberry, Antimicrob. Ag. Chemother. 15: 470 (1979).CrossRefGoogle Scholar
  69. 67.
    N. B. Shoemaker, M. D. Smith and W. R. Guild, J. Bacteriol. 139: 432 (1979).PubMedGoogle Scholar
  70. 68.
    F. Young, and L. Mayer, Rev. of Infect. Dis. 1: 55 (1979).CrossRefGoogle Scholar
  71. 69.
    A. Buu-hoi, and T. Horodniceanu, J. Bacteriol. 143: 313 (1980).PubMedGoogle Scholar
  72. 70.
    M. Smith and W. R. Guild, J. Bacteriol. 137: 735 (1979).PubMedGoogle Scholar
  73. 71.
    N. Shoemaker, M. Smith and W. Guild, Plasmid 3: 80 (1980).PubMedCrossRefGoogle Scholar
  74. 72.
    A. Franke and D. Clewell, Cold Spr. Harb. Symp. Quant. Biol. 45: in press (1980).Google Scholar
  75. 73.
    T. Horodniceanu, L. Bougueleret, and G. Bieth, Plasmid, in press (1981).Google Scholar
  76. 74.
    D. LeBlanc, This volumeGoogle Scholar
  77. 75.
    C. J. Smith, S. M. Markowitz, and F. Macrina, submitted for publication.Google Scholar
  78. 76.
    C. Gawron-Burke, A. Franke and D. B. Clewell, This volume.Google Scholar
  79. 77.
    J. F. Collins and P. Broda, Nature 258: 722 (1975).PubMedCrossRefGoogle Scholar
  80. 78.
    M. Bezdek, and J. Soska, Folia Microbiol. 17: 366 (1972).CrossRefGoogle Scholar
  81. 79.
    T. R. Manney and J. H. Meade, in: “Microbial Interaction (Series B, Receptors and Recognition, Vol. 3)”, J.L. Reissig, ed., Chapman and Hall, London (1977).Google Scholar
  82. 80.
    G. Kochert, Ann. Rev. Plant. Physiol. 29: 461 (1978).CrossRefGoogle Scholar
  83. 81.
    T. D. Mays, F. L. Macrina, R. A. Welch and C. J. Smith, This volume.Google Scholar
  84. 82.
    F. P. Tally, M. J. Shimell and M. H. Malamy, This volume.Google Scholar
  85. 83.
    D. Behnke and J. J. Ferretti, J. Bacteriol. 144: 806 (1980).PubMedGoogle Scholar
  86. 84.
    P. S. Lovett and K. M. Keggins, Methods in Enzymology 68: 342 (1979).PubMedCrossRefGoogle Scholar
  87. 85.
    D. Dubnau, T. Gryczan, S. Contente and A. G. Shivakumar, in: “Genetic Engineering”, Vol. 2, J. K. Setlow and A. Hollander, eds., Plenum, New York (1980).Google Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Don B. Clewell
    • 1
  1. 1.Depts. of Oral Biology Microbiology Schools of Dentistry and Medicine and the Dental Research InstituteUniversity of MichiganAnn ArborUSA

Personalised recommendations