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Cellulases: Diversity Amongst Improved Trichoderma Strains

  • S. P. Shoemaker
  • J. C. Raymond
  • R. Bruner
Part of the Basic Life Sciences book series

Abstract

In natural, mixed-culture fermentations of cellulose as well as in multistep conversion of cellulose to glucose, the depolymeriza-tion step seems rate limiting. Improvements in rate and yield of glucose from cellulose could greatly affect process economics (1–2). Indeed significant improvements have been achieved through pre-treatment (2–5), better strains (6–10), and process integration (11–12). As part of the Biomass Program at Cetus, we have attempted to increase the rate at which cellulose is utilized in fermentation processes by isolating organisms deregulated in the production of cellulase. For this work we have selected one of the most thoroughly studied of the cellulolytic organisms, Trichoderma reesei. This paper will focus on a few of our strains in order to illustrate their diversity both in the control of cellulase production and in the types and levels of the individual enzyme components. The data should give further support for the existence of multiple levels of control in Trichoderma. Finally, as a separate topic, cellulase productivity data will be presented for one of our best strains.

Keywords

High Performance Liquid Chromatography Cellulase Activity Cellulase Production Trichoderma Reesei Cotton Linter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • S. P. Shoemaker
    • 1
  • J. C. Raymond
    • 1
  • R. Bruner
    • 1
  1. 1.Cetus CorporationBerkeleyUSA

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