Advertisement

Induction and Regulation of Cellulase Synthesis in Trichoderma Mutants EA3 867 and N2-78

  • Y. S. Zhu
  • Y. Q. Wu
  • W. Chen
  • C. Tan
  • J. H. Gao
  • J. X. Fei
  • C. N. Shih
Part of the Basic Life Sciences book series

Abstract

Two mutants, EA30367 and N2-78 with high cellulase yields were obtained from wild strains of Trichoderma pseudokoningii Rifai, 1096 and MO3, respectively by mutagenic treatments with linear accelerator, 60Co, U.V., NTG, DTS, etc. The mutants showed small colonies on agar plates with synthetic medium, and grew slowly, but on agar plates with peptone-yeast extract medium the small colonies became as large as wild strains. The cellulase activities on these mutants in koji extracts, shake flask culture filtrates, and enzyme preparations were distinctly higher than those of their parents. The mutant N2-78 reached quite high activity level when cultured in shake flasks on a simple medium containing milled straw, wheat bran, nutrient salts plus waste glucose molasses for 60 hours, and showed the highest cellulase saccharifying activities on CMC, filter paper and cotton, namely 255, 82 and 13.4 mg glucose/ml enzyme respectively, or 11, 4.3 and 6 times more than those of its parent MO3.

Keywords

Wheat Bran Cellulase Activity Catabolite Repression Wild Strain Small Coloni 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Cellulase Research Laboratory, Shanghai Institute of Plant Physiology, Academia Cinica and the Shanghai Distillery No. 2: Isolation of two mutant stains of Trichoderma pseudokoningii Rifai EA3–867 and N2–78 with high cellulase yields and comparison of their characteristics, Acta Microbiological Sinican 18(1): 270–338, 1978.Google Scholar
  2. 2.
    Zhu, Y.S., and C. Tan: Induction and regulation of cellulose formation in Trichoderma I. the Induction of Cellulase formation in Trichoderma EA3–867 by sophorose. Acta Microbiologica Sinica 18(4) 320–331, 1978.Google Scholar
  3. 3.
    Zhu, Y.S. (Chu) and C. Tan: II. Induction of cellulase by sophorose in washed mycelia of T. pseudokoningii EA3–867 and its catabolite repression. Acta phytophysiologia Sinica 4(1): 19–26, 1978.Google Scholar
  4. 4.
    Zhu, Y.S. (Chu) and C. Tan: III. The inductive formation of cellulase by glucose waste molasses and extracts of pods of Sophora Japonica L. Acta Phytophysiologia Sinica 4(11): 19–26, 1978.Google Scholar
  5. 5.
    Zhu, Y.S. (Chu): IV. Changes in regulatory mechanisms of cellulase synthesis of two mutants with high yields. Acta Phytophysiologia Sinica 4(2): 143–151, 1978.Google Scholar
  6. 6.
    Wu, Y.Q., Y.S. Zhu, W. Chen, J.H. Gao and J.X. Fei: V. The changes in nucleic acid metabolism of washed mycelia of T. pseudokoningii Rafai N2–78 during inductive formation of cellulase. Acta Phytophysiologia Sinica. 5(4): 335–341.Google Scholar
  7. 7.
    Zhu, Y.S., Y.Q. Wu, J.H. Gao and J.X. Fei: VI. Separation, purification and properties of cellulase components of T. pseudokoningii Rafai N2–78 induced by sophorose, Acta Phytophysiologia Sinica 6(1): 1–9.Google Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Y. S. Zhu
    • 1
  • Y. Q. Wu
    • 1
  • W. Chen
    • 1
  • C. Tan
    • 1
  • J. H. Gao
    • 1
  • J. X. Fei
    • 1
  • C. N. Shih
    • 1
  1. 1.Shanghai Institute of Plant PhysiologyAcademia SinicaChina

Personalised recommendations