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Methanol and Carbon Monoxide Metabolism of Butyri-Bacterium Methylotrophicum the Marburg Strain

  • Lee H. Lynd
  • J. G. Zeikus
Part of the Basic Life Sciences book series

Abstract

Growth of B. methylotrophiewn on methanol (100mM)/acetate (50mM) required CO2 and exhibited: a chemical transformation stoichiometry of 10 methanol + 2CO2 → 3 butyrate: a cell yield of 7.8 g/mol methanol; and, a growth rate of 0.067 h-1. Cultures adapted to grow on methanol with a CO atmosphere (100%) displayed: a 3-fold increase in growth yield; increased growth rate; no requirement for CO2; and, production of acetate in lieu of butyrate. The maximum rate of CO oxidation displayed by cultures during mixotrophic growth on methanol/CO and 140 mmol/min/mg dry wt. B. methlotrophicum was adapted to grow in batch culture with a 100% CO atmosphere as the sole energy source. Fermentation parameters for growth on CO were: a chemical transformation stoichiometry of 4CO → 2.2 CO2 + 0.6 acetate; a growth yield of 2.9 g/mol CO; a final P.D.666 of 0.8; and, a maximal CO-oxidation rate of 260 nmol/min/mg dry wt. B-methylotrophicum grew on a variety of substrates in the presence of one atmosphere CO. Under these conditions, >.9 atmospheres of CO were consumed during growth on H2/CO2 or methanol, while <0.4 and <0.2 atmospheres were consumed during growth H2/CO2/CO increased greater than two-fold over that observed in the absence of CO.

Keywords

Acid Production Maximum Rate Batch Culture Butyric Acid Chemical Transformation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Lee H. Lynd
    • 1
  • J. G. Zeikus
    • 1
  1. 1.Department of BacteriologyUniversity of WisconsinMadisonUSA

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