Advertisement

Mineralogical and Biochemical Analysis of Lung Washing Fluid from Patients Exposed to Asbestos

  • Jean Bignon
  • Marie-Claude Jaurand
  • Patrick Sebastien
Part of the Ettore Majorana International Science Series book series (EMISS, volume 6)

Abstract

Recently, it has been shown in the baboon that broncho-alveolar lavage (BAL) is an efficient procedure for the recovery of radioactive particles deposited in the intra-alveolar compartment (1). For this reason, a prospective study was decided in order to investigate the possibility of assessing by means of this technique, the asbestos fiber content in the alveolar spaces of the human lung. The asbestos fibers recovered by BAL were quantitatively and qualitatively studied in order to see if this technique can help in the diagnosis of asbestos related diseases and if the fibers recovered are valuable indicators compared to sputum of past asbestos exposure and/of parenchymal asbestos burden.

Keywords

Alveolar Macrophage Sputum Sample Alveolar Space Asbestos Exposure Asbestos Fiber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    D. Nolibe, H. Metivier, R. Masse and J. Lafuma, Therapeutic effect of pulmonary lavage in vivo, after inhalation of insoluble radioactive particles. “Inhaled Particles” IV, W.H. Walton ed., Pergamon Press (1975)Google Scholar
  2. 2.
    E.G. Beck, P.F. Holt and N. Mandjlovic, Comparison of effects on macrophage cultures of glass fibers, glass powder and chrysotile asbestos. Br. J. Industr. Med., 29: 280 (1972).Google Scholar
  3. 3.
    P. Davies, A.C. Allison, J. Ackerman, A. Butterfield and S. Williams, Asbestos induces selective release of lysosomal enzymes from mononuclear phagocytes. Nature, 251: 423 (1974).PubMedCrossRefGoogle Scholar
  4. 4.
    M.C. Jaurand, J. Bignon, A. Gaudichet, L. Magne and A. Oblin, Biological effects of chrysotile after SO2 sorption. II. Effects on alveolar macrophages and red blood cells. Environ. Res. 17: 216 (1978).PubMedCrossRefGoogle Scholar
  5. 5.
    J.O. Harris, G.N. Olsen, J.R. Castle and A.S. Maloney, Comparison of proteolytic enzyme activity in pulmonary alveolar macrophages and blood leukocytes in smokers and nosmokers. Amer. Rev. Resp. Dis., 111: 579 (1975).PubMedGoogle Scholar
  6. 6.
    H.Y. Reynolds and H.H. Newball, Analysis of proteins and respiratory cells obtained from human lungs by bronchial lavage. J. Lab. Clin. Med., 84: 559 (1974).PubMedGoogle Scholar
  7. 7.
    G.A. Warr, R.R. Martin, P.M. Sharp and R.D. Rossen, Normal human bronchial immunoglobulins and proteins effects of cigarette smoking. Amer. Rev. Resp. Dis., 116: 25 (1977).PubMedGoogle Scholar
  8. 8.
    F. Basset, P. Soler, M.C. Jaurand and J. Bignon, Ultrastruc-tural examination of broncho-alveolar lavage for diagnosis of pulmonary histiocytosis X. Thorax, 32: 303 (1977).PubMedCrossRefGoogle Scholar
  9. 9.
    P. Sebastien, M.A. Billon, X. Janson, G. Bonnaud and J. Bignon, Utilisation du microscope électronique à transmission (MET) pour la mesure des contaminations par l’amiante. Arch. Mal. Prof., 39: 229 (1978).Google Scholar
  10. 10.
    J.W. Wollen, R. Heyworth and P.G, Walker, Studies on glyco-saminidase. Biochem. J., 78: 111 (1961).Google Scholar
  11. 11.
    P. Talalay, W.H. Fishman and C. Huggins, Chromogenic substrates. II. Phenolphtalein glucuronic acid as substrate for the assay of glucuronidase activity. J. Biol. Chem., 166: 757 (1946).PubMedGoogle Scholar
  12. 12.
    J. Conchie, J. Findlay and G.A. Lewy, Mammalian glycosidase. Distribution in the body. Biochem. J., 78: 111 (1961).Google Scholar
  13. 13.
    O.H. Lowry, N.J. Rosebrough, A.L. Farr and R. Randall, Protein measurement with the Folin phenal reagent. J. Biol. Chem. 193: 265 (1951).PubMedGoogle Scholar
  14. 14.
    J.C. Wagner, G. Berry, J.W. Skidmore and V. Timbrell, The effects of the inhalation of asbestos in rats. Br. J. Cancer, 29: 252 (1974).PubMedCrossRefGoogle Scholar
  15. 15.
    A. Morgan, J.C. Evans and A. Holmes, Deposition and clearance of inhaled fibrous minerals in the rat. Studies using radioactive tracer techniques. “Inhaled Particles” IV. W.H. Walton ed., Pergamon Press, Oxford (1977).Google Scholar
  16. 16.
    L. Le Bouffant, S. Bruyere, J.C. Martin, G. Tichoux and C. Normand, Quelques observations sur les fibres d’amiante et les formations minérales diverses rencon-trées dans les poumons asbestosiques. Rev. Fr. MalJ.Resp., 4: 121 (1976).Google Scholar
  17. 17.
    P. Sebastien, A. Fondimare, J. Bignon, G. Monchaux, J. Desbordes and G. Bonnaud, Topographic distribution of asbestos fibres in human lung in relation to occupational and non occupational exposure. “Inhaled Particles” IV. W.H. Walton ed., Pergamon Press, Oxford (1977).Google Scholar
  18. 18.
    A. Churg and M.L. Warnock, Analysis of the cores of ferruginous (asbestos) bodies from the General Population. I. Patients with and without lung cancer. Lab. Invest., 3: 280 (1977).Google Scholar
  19. 19.
    E.R. Weibel, Morphometry of the human lung. Heldeberg Springer Verlag (1963).Google Scholar
  20. 20.
    A. Morgan, R.J. Talboj and A. Holmes, Significance of fibre length in the clearance of asbestos fibres from the lung. Br. J. Industr. Med., 35: 146 (1978).Google Scholar
  21. 21.
    J. Bignon, G. Monchaux, A. Hirsch, P. Sebastien and J. Lafuma, Human and experimental data of translocation of asbestos fibers through the respiratory system. Ann. N.Y. Acad. Sci., 30: 745 (1979).CrossRefGoogle Scholar
  22. 22.
    M.R. Leadbertter and M. Corn, Particle size distribution of rat lung residues after exposure to fibre glass dust clouds. Am. Industr. Hyg. Assoc. J., 33: 511 (1972).CrossRefGoogle Scholar
  23. 23.
    M.C. Jaurand, A. Gaudichet, K. Atassi, P. Sebastien and J. Bignon, Relationship between the number of asbestos fibres and the cellular and enzymatic content of broncho-alveolar fluid in asbestos exposed subjects. Bull. Europ. Physio-Pathol. Resp. (in press).Google Scholar
  24. 24.
    R.R. Martin, Altered morphology and increased acid hydrolose content of pulmonary macrophages from cigarette smokers. Amer. Rev. Resp. Dis., 107: 596 (1973).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Jean Bignon
    • 1
  • Marie-Claude Jaurand
    • 1
  • Patrick Sebastien
    • 1
  1. 1.Service de PneumologieERA CNRS et LEPICréteil cedexFrance

Personalised recommendations