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GABA Release in Vivo and in Vitro: Responses to Physiological and Chemical Stimuli

  • Harry F. Bradford

Abstract

Studying the release of neurotransmitters from in vitro preparation presents special problems of possible artefact. The preparation of tissue slices, isolated ganglia, synaptosomes and their like always involves damaging tissue elements, whose partial or substantial recovery, usually by membrane-resealing, during subsequent incubation, is their limiting feature. It is unlikely that this recovery is ever complete and therefore the performance of such preparations, however sophisticated must be regarded with suspicion. In this respect amino acid neurotransmitters are a special case due to their ubiquitous presence in neural cells and their high concentrations (x 103) compared to other neurotransmitters. This renders more likely the possibility of artefact due to leakage through damaged membranes or diffusional loss down high concentration gradients enhanced by high fluid: tissue ratios. Where release is evoked by depolarizing agents such as veratrine or tityustoxin which stimulate active Na+ channels, and whose actions are entirely suppressed by tetrodotoxin, a much greater degree of confidence is engendered in the relevance of these transmitter release signals to the synaptic events which occur in the intact nervous system.

Keywords

Brachial Plexus Sensorimotor Cortex Gaba Release Gaba Uptake Amino Acid Release 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Abdul-Ghani, A.S., Bradford, H.F., Cox, D.W.G. and Dodd, P.R. Peripheral sensory stimulation and the release of transmitter amino acids in vivo from specific regions of cerebral cortex, Brain Res. 171 (1979) 55–66PubMedCrossRefGoogle Scholar
  2. 2.
    Abdul-Ghani, A.S., Coutinho-Netto, J. and Bradford, H.F., The action of,γ-vinyl GABA and γ-acetylenic GABA on the resting and stimulated release of GABA in vivo, Brain Res. (in press 1980).Google Scholar
  3. 3.
    Abdul-Ghani, A.S., Coutinho-Netto, J. and Bradford, H.F., In vivo release of acetylcholine evoked by brachial plexus stimulation and tityustoxin, Biochem. Pharmacol. 29 (1980) 2179–2182.PubMedCrossRefGoogle Scholar
  4. 4.
    Abdul-Ghani, A.S., Marton, M. and Dobkin, J., Studies of the transport of glutamine in vivo between the brain and blood in resting state and during afferent electrical stimulation, J. Neurochem. 31 (1978) 541–546.PubMedCrossRefGoogle Scholar
  5. 5.
    Abdul-Ghani, A.S., Norris, P.J., Smith, C.C.T. and Bradford, H.F., Effects of Y-acetylenic GABA and (-vinyl GABA on synaptosomal release and uptake of GABA, Biochem. Pharmacol. (in press 1980).Google Scholar
  6. 6.
    Albruz, K. and Herz, A., Inhibition of behavioural and EEG activation induced by morphine acting on lower brain-stem structures, Electroenc. Clin. Neurophysiol. 33 (1972) 579–590.CrossRefGoogle Scholar
  7. 7.
    Bradford, H.F., Bennett, G.W. and Thomas, A.J., Depolarizing stimuli and the release of physiologically active amino acids from suspension of mammalian synaptosomes, J. Neurochem. 21 (1973) 495–505.PubMedCrossRefGoogle Scholar
  8. 8.
    Bradford, H.F. and Thomas, A.J., Metabolism of glucose and glutamate by synaptosomes from mammalian cerebral cortex, J. Neurochem. 16 (1969) 1495–1504.PubMedCrossRefGoogle Scholar
  9. 9.
    Calvillo, 0., Henry, J.L. and Neuman, R.S., Effects of morphine and naloxone on dorsal horn neurones in the cat, Can. J. Physiol. Pharmac. 52 (1974) 1207–1211.CrossRefGoogle Scholar
  10. 10.
    Chang, K-J., Cooper, B.R., Hazum, E. and Cuatrecasas, P., Multiple opiate receptors: different regional distribution in the brain and differential binding of opiates and opioid peptides, Mol. Pharmacol. 16 (1979) 91–104.PubMedGoogle Scholar
  11. 11.
    Clark, R.M. and Collins, C.G.S., The release of endogenous amino acids from the rat visual cortex, J. Physiol. (Lond.), 262 (1976) 383–400.Google Scholar
  12. 12.
    Corrado, A.P. and Longo, V.G., An electrophysiological analysis of the convulsant action of morphine, codeine and thebaine, Arch. Int. Pharmacody. Ther. 132 (1961) 255.Google Scholar
  13. 13.
    Coutinho-Netto, J., Abdul-Ghani, A.S. and Bradford, H.F., Suppression of evoked and spontaneous release of neurotransmitters in vivo by morphine, Biochem. Pharmacol. (in press 1980).Google Scholar
  14. 14.
    Coutinho-Netto, J., Abdul-Ghani, A.S. Norris, P.J., Thomas, A. J. and Bradford, H.F., The effects of scorpion venom toxin on the release of amino acid neurotransmitters from cerebral cortex in vivo and in vitro, J. Neurochem. (in press 1980).Google Scholar
  15. 15.
    Countinho-Netto, J. and Diniz, C.R., 5th International Symposium on animal, plant and microbial toxins, (1976).Google Scholar
  16. 16.
    Denavit-Saubie, M., Champagnat, J. and Zieglgänsberger., Effects of opiates and methionine-enkephalin on pontine and bulbar respiratory neurones of the cat, Brain Res. 155 (1978) 55–67.PubMedCrossRefGoogle Scholar
  17. 17.
    Dingledine, R. and Goldstein, A., Single neuron studies of opiate action in the guinea pig myenteric plexus, Life Sciences 17 (1975) 57–62.PubMedCrossRefGoogle Scholar
  18. 18.
    Dodd, P.R. and Bradford, H.F., Reledse of amino acids from chronically superfused cerebral cortex, J. Neurochem. 23 (1974) 289–292.PubMedCrossRefGoogle Scholar
  19. 19.
    Dodd, P.R. and Bradford, H.F., Release of amino acids from the maturing cobalt-induced epileptic focus, Brain Res. 111 (1976) 377–388.PubMedCrossRefGoogle Scholar
  20. 20.
    Dodd, P.R., Pritchard, M.J. Adams, R.C.F., Bradford, H.F., Hicks, G. and Blanshard, K.C., A method for the continuous, long-term superfusion of the cerebral cortex of unanaesthetised, unrestrained rats, J. Scientific Inst. 7 (1974) 897–901.CrossRefGoogle Scholar
  21. 21.
    Dolly, J.0., Chun, K., Tse, V., Spokes, J.W. and Diniz, C.R., ß-bungarotoxin and tityustoxin on uptake and release of neurotransmitters, Biochem. Soc. Trans. 6 (1978) 652–654.PubMedGoogle Scholar
  22. 22.
    Dostrovsky, J. and Pomeranz, B., Morphine blockade of amino acid putative transmitters on cat spinal cord sensory interneurones, Nature New Biol. 246 (1973) 222.PubMedGoogle Scholar
  23. 23.
    Duggan, A.W., Hall, J.G. and Headley, P.M. Suppression of transmission of nociceptive impulses by morphine: selective effects of morphine administered in the region of the substantia gelatinosa, Br. J. Pharmac. 61 (1977) 65–76.Google Scholar
  24. 24.
    Dunlap, K. and Fischbach, G.D. Neurotransmitters decrease the calcium component of sensory neurone action potentials, Nature (Lond.) 276 (1978) 837–839.CrossRefGoogle Scholar
  25. 25.
    Fields, H.L., Emson, P.C., Leigh, B.I.C., Gilbert, R.F.T. and Iversen, L.L., Multiple opiate receptor sites on primary afferent fibres, Nature (Lond.) 284 (1980) 351–353.CrossRefGoogle Scholar
  26. 26.
    Frenk, H., McCarty, B.C., Liebeskind, J.C., Different brain areas mediate the analgesic and epileptic properties of enkephalin, Science 200 (1978) 335–337.PubMedCrossRefGoogle Scholar
  27. 27.
    Frenk, H., Urca, G. and Liebeskind, J.C., Epileptic properties of leucine-and methionine-enkephalin: comparison with morphine and reversibility by naloxone, Brain Res. 147 (1978) 327–337.PubMedCrossRefGoogle Scholar
  28. 28.
    Gray, E.G. and Whittaker, W.P., The isolation of nerve endings from brain, an electron microscopic study of cell fragments divided by homogenization and centrifugation, J. Anat. 96 (1962) 79–87.PubMedGoogle Scholar
  29. 29.
    Gudelsky, G.A. and Porter, J.C., Morphine and opioid peptide-induced inhibition of the release of dopamine from tuberoinfundibular neurons, Life Sciences 25 (1979) 1697.PubMedCrossRefGoogle Scholar
  30. 30.
    Iversen, L.L., Iversen, S.D. and Bloom, F.E., Opiate receptors influence vasopressin release from nerve terminals in rat neurohypophysis,Nature (Loud.) 284 (1980) 350–351.Google Scholar
  31. 31.
    Iversen, L.L., Mitchell, J.F. and Srinivasan, V., y-amino-butyric acid, during inhibition in the cat visual cortex. J. Physiol. (Lond.) 212 (1971) 519–534.Google Scholar
  32. 32.
    Jasper, H.H. and Koyama, I., Rate of release of amino acids from the cerebral cortex in the cat as affected by brain stem and thalamic stimulation, Canad. J. Physiol. Pharmacol. 47 (1969) 889–905.CrossRefGoogle Scholar
  33. 33.
    Jhamandas, K., Pinsky, C. and Phillis, J.W., Effects of morphine and its antagonists on release of cerebral cortical acetylcholine, Nature (Lond.) 288 (1970) 176.CrossRefGoogle Scholar
  34. 34.
    Jhamandas, K. and Sutak, M., Modification of brain acetylcholine release by morphine and its antagonists in normal and morphine-dependent rats, Br. J. Pharmac. 50 (1974) 57–62.Google Scholar
  35. 35.
    Jung, M.J., Lippert, B., Metcalfe, B.W., Böhlen, P. and Schechter, P.J., y-vinyl GABA (4-amino-hex–5-enoic acid), a new selective irreversible inhibitor of GABA-T: effects on brain GABA metabolism in mice J. Neurochem. 29 (1977) 797–802.PubMedCrossRefGoogle Scholar
  36. 36.
    Jung, M.J., Lippert, B., Metcalfe, B.W., Schechter, P.J., Böhlen, P. and Sjoerdsma, A., The effect of y-acetylenic GABA, catalytic inhibitor of GABA-T, on brain metabolism in vivo, J. Neurochem. 28 (1977) 717–723.CrossRefGoogle Scholar
  37. 37.
    Jung, M.J. and Metcalfe, B.W., Catalytic inhibition of GABA-T of cerebral origin by 4-amino-hex–5-ynoic acid. A substrate analog, Biochem. Biophys. Res. Commun. 67 (1975) 301–306.PubMedCrossRefGoogle Scholar
  38. 38.
    Jung, M.J. and Seiler, N., Enzyme-activated irreversible inhibitors of L-ornithine: 2-oxoacid aminotransferase, J. Biol. Chem. 253 (1978) 7431–7439.PubMedGoogle Scholar
  39. 39.
    Kaneto, H., in Narcotic Drugs: Biochemical Pharmacology (Ed. D. H. Clonet) (1971), p 300, Plenum Press, New YorkGoogle Scholar
  40. 40.
    Lamotte, C., Pert, C.B. and Snyder, S.S., Opiate receptor binding in primate spinal cord: distribution and changes after dorsal root section, Brain Res. 112 (1976) 407–412.PubMedCrossRefGoogle Scholar
  41. 41.
    Lippert, B., Metcalfe, B.W., Jung, M.J. and Casara, P., 4-aminohex–5-enoic acid, a selective catalytic inhibitor of GABA-T in mammalian brain, Europ. J. Biochem. 74 (1977) 441–445.PubMedCrossRefGoogle Scholar
  42. 42.
    Lowry, 0.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J., Protein measurement with the folín phenol reagent, J. Biol. Chem., 193 (1951) 265–275.PubMedGoogle Scholar
  43. 43.
    Matthews, J.D., Labrecque, G. and Domino, E.P., Effects of morphine, nalorphíne and naloxone on neocortícal release of acetylcholine in the rat, Psychopharmacología 29 (1973) 113–120PubMedCrossRefGoogle Scholar
  44. 44.
    Meldrum, B.S., Meniní, Ch., Stutzman, J.M. and Naquet, R., Effects of opiate-like peptides, morphine and naloxone in the photosensitive baboon, Papío papío, Brain Res. 170 (1979) 333–348.CrossRefGoogle Scholar
  45. 45.
    Metcalfe, B.W. and Casara, P., Region specific 1.4 addition of a proparglylic anion. A general synthon for 2-substantial propargylamine as potential catalytic irreversible enzyme inhibitors, Tetrahedron Lett. 38 (1975) 3337–3340.CrossRefGoogle Scholar
  46. 46.
    Mudge, A.W., Leeman, S.E. and Fischbach, G.D., Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration, Proc. Natl. Acad. Scí. U.S.A. 76 (1979) 526–530.PubMedCrossRefGoogle Scholar
  47. 47.
    Nicoll, R.A., Siggins, G.R., Ling, N., Bloom, F.E. and Guillemin, R., Ncurcnal actions of eudotpìlius and enkephalins among brain regions: a comparative mícroiontophoretic study, Proc. Nat. Acad. Sci. U.S.A. 74 (1977) 2584–2588.CrossRefGoogle Scholar
  48. 48.
    Norris, P.J., Smith, C.C.T., de Belleroche, J., Bradford, H.F., Mantle, P.G., Thomas, A.G. and Penney, R.H.C., Actions of tremorgenic fungal toxins on neurotransmitter release, J. Neurochem. 34 (1980) 33–42.PubMedCrossRefGoogle Scholar
  49. 49.
    North, R.A. and Tonini, M., The mechanism of action of narcotic analgesics in the guinea-pig ileum, Br. J. Pharmac. 61 (1977) 541–549.Google Scholar
  50. 50.
    Paton, W.D.M., The action of morphine and related substances on contraction and on acetylcholine output of coaxially stimulated guinea-pig ileum, Br. J. Chemother. 12 (1957) 119–127.Google Scholar
  51. 51.
    Perry, T.L., Kish, S.J., Sjaastrad, 0., Gjessing, L.R., Nesbakken, R., Schrader, H. and Loken, A.C., v-vinyl GABA: effects of chronic administration on the metabolism of GABA and other amino compounds in rat brain, J. Neurochem. 32 (1979) 1641–1645.PubMedCrossRefGoogle Scholar
  52. 52.
    Schaumann, W., Influence of atropine and morphine on the liberation of acetylcholine from the guinea pig’s intestine, Nature (Lond.) 178 (1956) 11–21.CrossRefGoogle Scholar
  53. 53.
    Schaumann, W., Inhibition by morphine of the release of acetylcholine from the intestine of the guinea-pig, Br. J. Pharmac. Chemother. 12 (1957) 115.Google Scholar
  54. 54.
    Takemori, A.C., Neurochemical bases for narcotic tolerance and dependence, Biochem. Pharmacol. 24 (1975) 2121–2125.PubMedCrossRefGoogle Scholar
  55. 55.
    Urca, G., Frenk, H., Liebeskínd, J.C. and Tailor, A.N., Morphine and enkephaline: analgesic and epileptic properties, Science 197 (1977) 83–86.PubMedCrossRefGoogle Scholar
  56. 56.
    Verdeaux, G. and Manty, R., Action sur l’électroencéphalogramme de substances pharmacodynamiques d’interet clinique, Rev. Neurol. 91 (1954) 405–427.Google Scholar
  57. 57.
    Weinstock, M., in Narcotic Drugs: Biochemical Pharmacology(Ed. D.H. Clonet) (1971), p 254, Plenum Press, New York.Google Scholar
  58. 58.
    Westerfield, M., Moore, J.W., Kim, Y. S. and Padilla, G.M., How Gymnodinium breve red tide toxin(s) produces repetitive firing in squid axons, Am. J. Physiol. 232 (1977) C23- C29.PubMedGoogle Scholar
  59. 59.
    Wourters, W. and Van den Bercken, J., Hyperpolarisation and depression of slow synaptic inhibition by enkephalin in frog sympathetic ganglion, Nature, (Lond.) 277 (1979) 53.CrossRefGoogle Scholar
  60. 60.
    Zieglgänsberger, W. and Bayerl, H., The mechanism of inhibition of neuronal activity by opiates in the spinal cord of cat, Brain Res. 115 (1976) 111–128.PubMedCrossRefGoogle Scholar
  61. 61.
    Zieglgänsberger, W., Fry, J.P., Herz, A., Moroder, L. and Wünsch, E., Enkephalin-induced inhibition of cortical neurones and the lack of this effect in morphine tolerant/dependent rats, Brain Res. 115 (1976) 160.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Harry F. Bradford
    • 1
  1. 1.Department of BiochemistryImperial CollegeLondonEngland

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