Vacuolar Nitrate and the Isolation of Vacuoles for Localization and Transport Studies
The vacuole is presumed to be the site of nitrate storage in plants. Various authors (Heimer and Filner, 1971; Ferrari et al., 1973; and Aslam et al., 1976) have demonstrated the existence of an intracellular pool from which nitrate can be made available for reduction. Washout kinetics obtained from tobacco suspension cells indicate that this pool may represent vacuolar nitrate (Ferrari et al., 1973). However, no direct evidence exists that the vacuole is indeed the main storage area of cellular nitrate.
KeywordsAcid Phosphatase Activity Acid Invertase Tobacco Suspension Cell Flotation System Fraction Label
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