Vacuolar Nitrate and the Isolation of Vacuoles for Localization and Transport Studies

  • Robert C. Granstedt
  • R. C. Huffaker


The vacuole is presumed to be the site of nitrate storage in plants. Various authors (Heimer and Filner, 1971; Ferrari et al., 1973; and Aslam et al., 1976) have demonstrated the existence of an intracellular pool from which nitrate can be made available for reduction. Washout kinetics obtained from tobacco suspension cells indicate that this pool may represent vacuolar nitrate (Ferrari et al., 1973). However, no direct evidence exists that the vacuole is indeed the main storage area of cellular nitrate.


Acid Phosphatase Activity Acid Invertase Tobacco Suspension Cell Flotation System Fraction Label 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Robert C. Granstedt
    • 1
  • R. C. Huffaker
    • 1
  1. 1.Plant Growth Laboratory and the Department of Agronomy & Range ScienceUniversity of CaliforniaDavisUSA

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