Interaction Between \(N{O_3}^ - \), \(N{O_2}^ - \), and \(N{H_4}^ + \) During Assimilation in Detached Barley Leaves

  • S. S. Goyal
  • R. C. Huffaker

Abstract

Numerous reports show that plants may utilize both NO3 and NH4 + ions as a source of nitrogen. In microorganisms, ammonium often feedback-inhibits NR activity and represses NR induction (Payne, 1973). Because NO3 reduction seems to occur in the cytoplasm (Beevers and Hageman, 1972; Neyra and Hageman, 1978) and NO2 and NH4 + are assimilated in the chloroplast, feedback-inhibition by products may not be as serious in green leaves. Little information is available concerning the effects of each of these nitrogenous compounds on the assimilation of the others. This paper deals with that problem.

Keywords

Sucrose HPLC Dioxide Corn Germinate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aslam, M., Huffaker, R. C., Rains, D. W., and Rao, K. Prasad, 1979, Influence of light and ambient carbon dioxide concentration on nitrate assimilation by intact barley seedlings, Plant Physiol., 63:1025.CrossRefGoogle Scholar
  2. Baily, L. J., 1962, Techniques in Protein Chemistry, Elsevier, Amsterdam, 299.Google Scholar
  3. Beevers, L., and Hageman, R. H., 1972, The role of light in nitrate metabolism in higher plants, in: “Photophysiology,” Vol. 7, Chap. 4, A. C. Giese, ed., Academic Press, New York.Google Scholar
  4. Bennet, W. F., Pesck, J., and Hanway, J. J., 1964, Effect of NO3 and NH4 + on growth of corn in nutrient solution sand culture, Agron. J., 56:342.CrossRefGoogle Scholar
  5. Breteler, H., and Smit, A. L., 1974, Effect of ammonium nutrition on uptake and metabolism of nitrate in wheat, Neth. J. Agric. Sci., 22:73.Google Scholar
  6. Erickson, T., 1965, Studies on the growth requirements and growth measurements of cell cultures of Haplopappus gracilis, Physiol. Plant., 18:976.CrossRefGoogle Scholar
  7. Gamborg, O. L., Miller, R. A., and Ojima, K., 1968, Nutrient requirements of suspension cultures of soybean root cells, Exp. Cell Res., 50:151.PubMedCrossRefGoogle Scholar
  8. Goyal, S. S., 1974, Studies on the inhibitory effects of ammoniacal nitrogen on growth of radish plants (Raphanus sativus) and its reversal by nitrate, Ph.D. Dissertation, University of California, Davis.Google Scholar
  9. Hoagland, D. R., and Arnon, D. I., 1950, The water culture method for growing plants without soil, Calif. Agric. Exp. Sta. Circ. No. 347.Google Scholar
  10. Neyra, C. A., and Hageman, R. H., 1978, Pathway of nitrate assimilation in corn (Zea mays L.) leaves. Cellular distribution of enzymes and energy sources for nitrate reduction, Plant Physiol., 62:618.PubMedCrossRefGoogle Scholar
  11. Oaks, A., Stulen, I., and Boesel, I. L., 1979, Influence of amino acids and ammonium on nitrate reduction in corn seedlings, Can. J. Bot., 57:1824.CrossRefGoogle Scholar
  12. Payne, W. J., 1973, Reduction of nitrogenous oxides by microorganisms, Bacteriol. Rev., 37:429.Google Scholar
  13. Schrader, L. E., and Hageman, R. H., 1967, Regulation of nitrate reductase in corn seedlings by endogenous metabolites, Plant Physiol., 42:1750.PubMedCrossRefGoogle Scholar
  14. Schrader, L. E., Domska, D., Jung, Jr., P. E., and Peterson, L. A., 1972, Uptake and assimilation of ammonium-N and nitrate-N and their influence on the growth of corn (Zea mays L.), Agron J., 64:690.CrossRefGoogle Scholar
  15. Sihag, R. K., Guha-Mukherjee, S., and Sopory, S. K., 1979, Effect of ammonium, sucrose, and light on the regulation of nitrate reductase level in Pisum sativum, Physiol. Plant., 45:281.CrossRefGoogle Scholar
  16. Thayer, J. R., and Huffaker, R. C., 1980, Determination of nitrate and nitrite by high-pressure liquid chromatography: comparison with other methods for nitrate determination, Anal. Biochem., 102:110.PubMedCrossRefGoogle Scholar
  17. Wahhab, A., and Bhati, H. M., 1957, Effect of various sources of nitrogen on rice paddy yields, Agron. J., 49:114.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • S. S. Goyal
    • 1
  • R. C. Huffaker
    • 1
  1. 1.Plant Growth Laboratory and the Department of Agronomy and Range ScienceUniversity of CaliforniaDavisUSA

Personalised recommendations