Light Interaction with Nitrate Reduction

  • Steven L. Morrison
  • R. C. Huffaker
  • C. Rick Guidara


Light affects assimilation of nitrate via nitrate reductase in many ways. Two prominent examples are: an effect on uptake and movement of nitrate between storage and metabolic pools; and production and distribution of reducing power and generation of carbon skeletons via photosynthesis. Nitrate reductase activity (NRA) correlates more closely with nitrate flux into leaf tissue than leaf nitrate concentration. The possibility that nitrate “flux” through a pool may regulate NRA fits well with the postulation that NO3 exists in “metabolic” (active) pools, and “storage” (inactive) pools, where only the active pool is available for induction of NRA and for use as a substrate. The existence of “storage” and “metabolic” pools has been demonstrated in tobacco cells (Heimer and Filner, 1971; Ferrari et al., 1973), corn roots (Aslam and Oaks, 1975) and etiolated barley leaves (Aslam et al., 1976).


Nitrate Reductase Nitrate Reduction Nitrate Reductase Activity Nitrate Uptake Primary Leaf 
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  1. Aslam, M., Huffaker, R. C., Rains, D. W., and Rao, K. P., 1979, Influence of light and ambient carbon dioxide concentration on nitrate assimilation by intact barley seedlings, Plant Physiol., 63:1205.PubMedCrossRefGoogle Scholar
  2. Aslam, M., and Oaks, A., 1975, Effect of glucose on the induction of nitrate reductase in corn roots, Plant Physiol., 56:634.PubMedCrossRefGoogle Scholar
  3. Aslam, M., Oaks, A., and Huffaker, R. C., 1976, Effect of light and glucose on the induction of nitrate reductase and on the distribution of nitrate in etiolated barley leaves, Plant Physiol., 58:588.PubMedCrossRefGoogle Scholar
  4. Beevers, L., and Hageman, R. H., 1969, Nitrate reduction in higher plants, Ann. Rev. Plant Physiol., 20:495.CrossRefGoogle Scholar
  5. Canvin, D. T., and Atkins, C. A., 1974, Nitrate, nitrite and ammonia assimilation by leaves: effect of light, carbon dioxide and oxygen, Planta (Berl.), 116:207.CrossRefGoogle Scholar
  6. Chantarotwong, W., Huffaker, R. C., Miller, B. L., and Granstedt, R. C., 1976, In vivo nitrate reduction in relation to nitrate uptake, nitrate content, and in vitro nitrate reductase activity in intact barley seedlings, Plant Physiol., 57:519.PubMedCrossRefGoogle Scholar
  7. Dalling, M. J., Tolbert, N. E., and Hageman, R. H., 1972, Intracellular location of nitrate reductase and nitrite reductase. I. Spinach and tobacco leaves, Biochim. Biophys. Acta, 238:505.Google Scholar
  8. Ferrari, T. E., Yoder, O. C., and Filner, P., 1973, Anaerobic nitrite production by plant cells and tissues: evidence for two nitrate pools, Plant Physiol., 51:423.PubMedCrossRefGoogle Scholar
  9. Grant, B. R., Atkins, C. A., and Canvin, D. T., 1970, Intracellular location of nitrate reductase and nitrite reductase in spinach and sunflower leaves, Planta, 94:60.CrossRefGoogle Scholar
  10. Heimer, Y. M., and Filner, P., 1971, Regulation of the nitrate assimilation pathway in cultured tobacco cells. III. The nitrate uptake system, Biochim. Biophys. Acta, 230:362.PubMedCrossRefGoogle Scholar
  11. Heber, U., and Willenbrink, J., 1964, Sites of synthesis and transport of photosynthetic products within the leaf cell, Biochim. Biophys. Acta, 82:313.PubMedCrossRefGoogle Scholar
  12. Ito, O., Yoneyama, T., and Kumazawa, K., 1978, Amino acid metabolism in plant leaf. IV. The effect of light in ammonium assimilation and glutamine metabolism in the cells isolated from spinach leaves, Plant and Cell Physiol., 19:1109.Google Scholar
  13. Klepper, L. A., Flesher, D., and Hageman, R. H., 1971, Generation of reduced NAD for nitrate reduction in green leaves, Plant Physiol., 48:580.PubMedCrossRefGoogle Scholar
  14. Klepper, L. A., and Hageman, R. H., 1969, Dependence of nitrate reduction on generation of reduced nicotinamide adenine dinucleotide by glycolytic metabolism of leaf tissue, Fed. Proc. Amer. Soc. Exp. Biol., 28:467.Google Scholar
  15. Rains, D. W., 1968, Kinetics and energetics of light enhanced potassium absorption by corn leaf tissue, Plant Physiol., 43:394.PubMedCrossRefGoogle Scholar
  16. Ritenour, G. L., Joy, K. W., Bunning, J., and Hageman, R. H., 1967, Intracellular localization of nitrate reductase and glutamic acid dehydrogenase in green leaf tissue, Plant Physiol., 42:233.PubMedCrossRefGoogle Scholar
  17. Sawhney, S. K., Naik, M. S., and Nicholas, D. J. D., 1978, Regulation of nitrate reduction by light, ATP, and mitochondrial respiration in wheat leaves, Nature, 272:647.CrossRefGoogle Scholar
  18. Shaner, D. L., and Boyer, J. S., 1976a, Nitrate reductase activity in maize (Zea mays L.) leaves. I. Nitrate regulation by nitrate flux, Plant Physiol., 58:499.PubMedCrossRefGoogle Scholar
  19. Shaner, D. L., and Boyer, J. S., 1976b, Nitrate reductase activity in maize (Zea mays L.) leaves. II. Regulation by nitrate flux at low leaf water potential, Plant Physiol., 58:505.PubMedCrossRefGoogle Scholar
  20. Sherrard, J. H., and Hageman, R. H., 1980, The influence of oxygen on nitrate reductase in wheat leaves in relation to mitochondrial activity, Plant Physiol., S:289.Google Scholar
  21. Smith, R. C., and Epstein, E., 1964, Ion absorption by shoot tissue: technique and first findings with excised leaf tissue of corn, Plant Physiol., 39:338.PubMedCrossRefGoogle Scholar
  22. Stocking, C. R., and Larson, S., 1969, A chloroplast cytoplasmic shuttle and the reduction of extraplastid NAD, Biochem. Biophys. Res. Commun., 37:278.PubMedCrossRefGoogle Scholar
  23. Stocking, C. R., Williams, G. R., and Ongun, A., 1963, Intracellular distribution of the early products of photosynthesis, Biochem. Biophys. Res. Commun., 10:416.PubMedCrossRefGoogle Scholar
  24. Swader, J. A., and Stocking, C. R., 1971, Nitrate and nitrite reduction by Wolffia arrhiza, Plant Physiol., 47:189.PubMedCrossRefGoogle Scholar
  25. Thayer, J. R., and Huffaker, R. C., 1980, Determination of nitrate and nitrite by high-pressure liquid chromatography: comparison with other methods for nitrate determination, Anal. Biochem., 102:110.PubMedCrossRefGoogle Scholar
  26. Urbach, W., Hudson, M. A., Ulrich, W., Santarius, K. A., and Heber, U., 1965, Verteilung und Wanderung von phosphoglycerat zwischen den chloroplasts und dem zytoplasm während der photosynthese, Z. Naturforsch. B., 20:890.PubMedGoogle Scholar
  27. Walker, D. A., and Crofts, A. R., 1970, Photosynthesis, Ann. Rev. Biochem., 39:389.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Steven L. Morrison
    • 1
  • R. C. Huffaker
    • 1
  • C. Rick Guidara
    • 1
  1. 1.Plant Growth Laboratory and the Department of Agronomy & Range ScienceUniversity of CaliforniaDavisUSA

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