Molecular Cloning of Nitrogen Fixation Genes from Rhizobium Meliloti

  • Frederick M. Ausubel
  • Gary B. Ruvkun


Until recently, the focus of interest in our laboratory has been on cloning nif genes from the free-living nitrogen-fixing species Klebsiella pneumoniae (Cannon et al., 1977; Cannon et al., 1979; Riedel et al., 1979). K. pneumoniae was chosen for the first attempts to clone nif genes because its close resemblance to Escherichia coli allowed it to be directly studied by E. coli genetic techniques.


Nitrogen Fixation Klebsiella Pneumoniae EcoRI Fragment Symbiotic Nitrogen Fixation Nitrogen Fixation Gene 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Frederick M. Ausubel
    • 1
  • Gary B. Ruvkun
    • 1
  1. 1.Cellular and Developmental Biology Group Department of BiologyBiological Laboratories Harvard UniversityCambridgeUSA

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