The Conversion of \(N{O_3}^ - \) to \(N{H_4}^ + \) in Klebsiella Pneumoniae

  • J. R. Thayer
  • R. C. Huffaker


In soil environments several microbial oxidations and reductions of nitrogen (N) may occur depending on the conditions. Figure 1 shows those of interest to this study. Anaerobically, NO3 is reduced to NO2 , which can be converted to gaseous forms and the N lost, or may be converted to NH4 + and assimilated or excreted and the N conserved through interaction with soil particles.


Nitrogen Fixation Nitrate Reductase Klebsiella Pneumoniae Nitrate Transport Bacterial Respiration 


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  1. Alexander, M., 1977, An Introduction to Soil Microbiology, 2nd Ed., John Wiley & Sons, New York.Google Scholar
  2. Andersen, K., and Shanmugam, K. T., 1977, Energetics of biological nitrogen fixation: determination of the ratio of formation of H2 to NH4 + catalyzed by nitrogenase of Klebsiella pneumoniae in vivo, J. Gen. Microbiol., 103:107.PubMedGoogle Scholar
  3. Cohen-Bazire, G., Sistrom, W. R., and Stanier, R. Y., 1957, Kinetic studies of pigment synthesis by non-sulphur bacteria, J. Cell Comp. Physiol., 49:25.CrossRefGoogle Scholar
  4. Garland, P. B., Downie, J. A., and Haddock, B. A., 1975, Proton translocation and respiratory nitrate reductase of Escherichia coli, Biochem. J., 152:549.Google Scholar
  5. Haddock, B. A., and Jones, C. W., 1977, Bacterial respiration, Bacteriol. Rev., 41:47.PubMedGoogle Scholar
  6. Herbert, R. A., 1980, Nitrogen cycling, with particular reference to nitrate dissimilation in estuarine and marine sediments, Abstracts of the Symposium on the Microbiology of Sediments at Cambridge, March 1980, Soc. Gen. Microbiol. Quart., 7:65.Google Scholar
  7. Horn, S. S. M., Hennecke, H., and Shanmugam, K. T., 1980, Regulation of nitrogenase biosynthesis in Klebsiella pneumoniae: effect of nitrate, J. Gen. Microbiol., 117:169.Google Scholar
  8. John, P., 1977, Aerobic and anaerobic bacterial respiration monitored by electrodes, J. Gen. Microbiol., 98:231.PubMedGoogle Scholar
  9. Kashket, E. R., and Barker, S. L., 1977, Effects of potassium ions on the electrical and pH gradients across the membranes of Streptococcus lactis cells, J. Bacteriol., 130:1017.PubMedGoogle Scholar
  10. Kristajansson, J. K., and Hollocher, T. C., 1979, Substrate binding site for nitrate reductase of Escherichia coli is on the inner aspect of the membrane, J. Bacteriol., 137:1227.Google Scholar
  11. Kristajansson, J. K., Walter, B., and Hollocher, T. C., 1978, Respiration-dependent proton translocation and the transport of nitrate and nitrite in Paracoccus denitrificans and other denitrifying bacteria, Biochemistry, 17:5014.CrossRefGoogle Scholar
  12. Parks, N. J., and Krohn, K. A., 1978, The synthesis of 13N labeled ammonia, dinitrogen, nitrite, and nitrate using a single cyclotron target system, Int. J. Appl. Radiat. Isot., 29:754.CrossRefGoogle Scholar
  13. Schrader, L. E., Ritenour, G. L., Elrich, G. L., and Hageman, R. H., 1968, Some characteristics of nitrate reductase from higher plants, Plant Physiol., 43:930.PubMedCrossRefGoogle Scholar
  14. Shanmugam, K. T., Chan, I., and Morandi, C., 1975, Regulation of nitrogen fixation. Nitrogenase-derepressed mutants of Klebsiella pneumoniae, Biochim. Biophys. Acta, 408:101.PubMedCrossRefGoogle Scholar
  15. Sias, S. R., and Ingraham, J., 1979, Isolation and analysis of mutants of Pseudomonas aeruginosa unable to assimilate nitrate, Arch. Microbiol., 122:263.PubMedCrossRefGoogle Scholar
  16. Thayer, J. R., and Huffaker, R. C., 1980, Determination of nitrate and nitrite by high-pressure liquid chromatography. Comparison with other methods, Anal. Biochem., 102:110.PubMedCrossRefGoogle Scholar
  17. Thayer, J. R., and Huffaker, R. C., 1981, Use of l3N-nitrate to study nitrate transport in Klebsiella pneumoniae, in: “Short-Lived Radionuclides in Chemistry and Biology,” K. A. Krohn and J. W. Root, eds., Advances in Chemistry Series, Washington, D. C., American Chemical Society (in press).Google Scholar
  18. Tiedje, J. M., Firestone, M. K., Betlach, M. R., Smith, M. S., and Caskey, W. H., 1979, Methods for the production and use of nitrogen-13 in studies of denitrification, Soil Sci. Soc. Am. J., 43:709.CrossRefGoogle Scholar
  19. Van’t Riet, J., Stouthamer, A. H., and Planta, R. J., 1968, Regulation of nitrate assimilation and nitrate respiration in Aerobacter aerogenes, J. Bacteriol., 96:1455.Google Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • J. R. Thayer
    • 1
  • R. C. Huffaker
    • 1
  1. 1.Plant Growth Laboratory and the Department of Agronomy & Range ScienceUniversity of CaliforniaDavisUSA

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